Real flue gas CO2 hydrogenation to formate by an enzymatic reactor using O2- and CO-tolerant hydrogenase and formate dehydrogenase

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Jinhee Lee¹  Byoung Wook Jeon¹  Yong Hwan Kim¹  Jaehyun Cha²  Inchan Kwon³ 

[1] School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, Republic of Korea;School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea;School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea;Research Center for Innovative Energy and Carbon Optimized Synthesis for Chemicals (Inn-ECOSysChem), Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea;

关键词: carbon dioxide;    hydrogen;    formate;    flue gas;    hydrogenase;    formate dehydrogenase;   

DOI  :  10.3389/fbioe.2023.1265272

 received in 2023-07-22, accepted in 2023-09-22,  发布年份 2023

来源: Frontiers

【 摘 要 】

It is challenging to capture carbon dioxide (CO2), a major greenhouse gas in the atmosphere, due to its high chemical stability. One potential practical solution to eliminate CO2 is to convert CO2 into formate using hydrogen (H2) (CO2 hydrogenation), which can be accomplished with inexpensive hydrogen from sustainable sources. While industrial flue gas could provide an adequate source of hydrogen, a suitable catalyst is needed that can tolerate other gas components, such as carbon monoxide (CO) and oxygen (O2), potential inhibitors. Our proposed CO2 hydrogenation system uses the hydrogenase derived from Ralstonia eutropha H16 (ReSH) and formate dehydrogenase derived from Methylobacterium extorquens AM1 (MeFDH1). Both enzymes are tolerant to CO and O2, which are typical inhibitors of metalloenzymes found in flue gas. We have successfully demonstrated that combining ReSH- and MeFDH1-immobilized resins can convert H2 and CO2 in real flue gas to formate via a nicotinamide adenine dinucleotide-dependent cascade reaction. We anticipated that this enzyme system would enable the utilization of diverse H2 and CO2 sources, including waste gases, biomass, and gasified plastics.

【 授权许可】

Unknown   
Copyright © 2023 Cha, Lee, Jeon, Kim and Kwon.