Methotrexate-conjugated zinc oxide nanoparticles exert a substantially improved cytotoxic effect on lung cancer cells by inducing apoptosis

Frontiers in Pharmacology

Pharmacology

Lamya Ahmed Al-Keridis¹  Nadiyah M. Alabdallah²  Nawaf Alshammari³  Mohd Saeed³  Mahima Verma⁴  Afza Ahmad⁴  Shireen Fatima⁴  Irfan Ahmad Ansari⁴  Prakriti Mishra⁴  Mohd Faizan Ali Ahmad⁴  Rohit Kumar Tiwari⁵ 

[1] Biology Department, Faculty of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia;Department of Biology, College of Science, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia;Basic and Applied Scientific Research Centre, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia;Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia;Department of Biosciences Integral University Lucknow, Lucknow, India;Department of Biosciences Integral University Lucknow, Lucknow, India;Department of Clinical Research, School of Allied Health Sciences, Sharda University, Uttar Pradesh, India;

关键词: zinc oxide;    nanoparticles;    methotrexate;    lung cancer;    A549;    reactive oxygen species;    caspases;   

DOI  :  10.3389/fphar.2023.1194578

 received in 2023-03-27, accepted in 2023-09-22,  发布年份 2023

来源: Frontiers

【 摘 要 】

In the current study, we report the synthesis of methotrexate-conjugated zinc oxide nanoparticles (MTX-ZnONPs) and their high efficacy against lung cancer cells. Conjugation of MTX with ZnONPs was authenticated by UV-vis spectroscopy, dynamic light scattering (DLS), Fourier-transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM). This drug-nanoconjugate also showed high drug-loading efficiency. The therapeutic efficacy of MTX-ZnONPs was further tested in vitro against A549 cells, and the results of MTT and LDH release assays showed that MTX-ZnONPs, in addition to free MTX, were efficient in exerting cytotoxic effect on A549 cells; however, the effectiveness of MTX-ZnONPs was found to be considerably enhanced at very low doses compared to that of free MTX. Moreover, ZnONPs alone significantly inhibited the cell viability of A549 cells at a much higher concentration compared to MTX-ZnONPs and MTX. Furthermore, the cytomorphology of A549 cells was characterized by cellular shrinkage and detachment from the surface in all the treatment groups. Similarly, A549 cells, in all the treatment groups, showed fragmented and condensed nuclei, indicating the initiation of apoptosis. Mitochondrial membrane potential (ψm) in A549 cells showed a gradual loss in all the treatment groups. Results of the qualitative and quantitative analyses depicted increased reactive oxygen species (ROS) levels in A549 cells. The results of the caspase activity assay showed that MTX-ZnONPs andfree MTX caused significant activation of caspase-9, -8, and -3 in A549 cells; however, the effect of MTX-ZnONPs was more profound at very low doses compared to that of free MTX. Thus, our results showed high efficacy of MTX-ZnONPs, suggesting efficient intracellular delivery of the drug by ZnONPs as nanocarriers.

【 授权许可】

Unknown   
Copyright © 2023 Mishra, Ali Ahmad, Al-Keridis, Saeed, Alshammari, Alabdallah, Tiwari, Ahmad, Verma, Fatima and Ansari.