Oral exposure to Staphylococcus aureus enterotoxin B could promote the Ovalbumin-induced food allergy by enhancing the activation of DCs and T cells

Frontiers in Immunology

Immunology

Jinyan Gao¹  Xuanyi Meng²  Yong Wu²  Ping Tong³  Xin Li⁴  Hongbing Chen⁵  Jin Yuan⁶ 

[1] College of Food Science & Technology, Nanchang University, Nanchang, China;Sino-German Joint Research Institute, Nanchang University, Nanchang, China;State Key Laboratory of Food Science and Resource, Nanchang University, Nanchang, China;State Key Laboratory of Food Science and Resource, Nanchang University, Nanchang, China;College of Food Science & Technology, Nanchang University, Nanchang, China;State Key Laboratory of Food Science and Resource, Nanchang University, Nanchang, China;Sino-German Joint Research Institute, Nanchang University, Nanchang, China;State Key Laboratory of Food Science and Resource, Nanchang University, Nanchang, China;Sino-German Joint Research Institute, Nanchang University, Nanchang, China;College of Food Science & Technology, Nanchang University, Nanchang, China;

关键词: Staphylococcus aureus enterotoxin B;    food allergy;    dendritic cells;    T cells;    gut microbiota;   

DOI  :  10.3389/fimmu.2023.1250458

 received in 2023-07-25, accepted in 2023-09-29,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionRecent work highlighted the importance of environmental contaminants in the development of allergic diseases.MethodsThe intestinal mucosal barrier, Th (helper T) cells, DCs (dendritic cells), and intestinal flora were analyzed with flow cytometry, RNA-seq, and 16s sequencing in the present study to demonstrate whether the exposure of enterotoxins like Staphylococcus aureus enterotoxin B (SEB) in allergens could promote the development of food allergy.Results and discussionWe found that co-exposure to SEB and Ovalbumin (OVA) could impair the intestinal barrier, imbalance the intestinal Th immune, and cause the decline of intestinal flora diversity in OVA-sensitized mice. Moreover, with the co-stimulation of SEB, the transport of OVA was enhanced in the Caco-2 cell monolayer, the uptake and presentation of OVA were promoted in the bone marrow dendritic cells (BMDCs), and Th cell differentiation was also enhanced. In summary, co-exposure to SEB in allergens should be considered a food allergy risk factor.

【 授权许可】

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Copyright © 2023 Yuan, Tong, Meng, Wu, Li, Gao and Chen