Development of an acute ovine model of polycystic ovaries to assess the effect of ovarian denervation

Frontiers in Endocrinology

Endocrinology

Bruce K. Campbell¹  Jason Witherington²  Jason A. Miranda²  W. Colin Duncan³  Linda M. Nicol³  Rosie O’Hare³  Jennifer L. Thomas⁴  Michael T. Rae⁴ 

[1] Division of Child Health, Obstetrics and Gynaecology, The University of Nottingham, Nottingham, United Kingdom;Galvani Bioelectronics, Stevenage, United Kingdom;MRC Centre for Reproductive Health, The University of Edinburgh, Edinburgh, United Kingdom;School of Applied Sciences, Edinburgh Napier University, Edinburgh, United Kingdom;

关键词: follicle;    tyrosine hydroxylase;    polycystic ovary syndrome;    sympathetic nerve;    gonadotrophin;   

DOI  :  10.3389/fendo.2023.1285269

 received in 2023-08-29, accepted in 2023-10-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionPolycystic ovary syndrome (PCOS) seems to be associated with increased ovarian sympathetic nerve activity and in rodent models of PCOS reducing the sympathetic drive to the ovary, through denervation or neuromodulation, improves ovulation rate. We hypothesised that sympathetic nerves work with gonadotropins to promote development and survival of small antral follicles to develop a polycystic ovary phenotype.MethodsUsing a clinically realistic ovine model we showed a rich sympathetic innervation to the normal ovary and reinnervation after ovarian transplantation. Using needlepoint diathermy to the nerve plexus in the ovarian vascular pedicle we were able to denervate the ovary resulting in reduced intraovarian noradrenaline and tyrosine hydroxylase immunostained sympathetic nerves. We developed an acute polycystic ovary (PCO) model using gonadotrophin releasing hormone (GnRH) agonist followed infusion of follicle stimulating hormone (FSH) with increased pulsatile luteinising hormone (LH). This resulted in increased numbers of smaller antral follicles in the ovary when compared to FSH infusion suggesting a polycystic ovary.ResultsDenervation had no effect of the survival or numbers of follicles in the acute PCO model and did not impact on ovulation, follicular and luteal hormone profiles in a normal cycle.DiscussionAlthough the ovary is richly inervated we did not find evidence for a role of sympathetic nerves in ovarian function or small follicle growth and survival.

【 授权许可】

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Copyright © 2023 Duncan, Nicol, O’Hare, Witherington, Miranda, Campbell, Thomas and Rae