Impact of a booster dose on SARS-CoV2 mRNA vaccine-specific humoral-, B- and T cell immunity in pediatric stem cell transplant recipients

Frontiers in Immunology

Immunology

Arne Sattler¹  Katja Kotsch¹  Carolin Stahl¹  Janine Siegle¹  Laura Tóth¹  Vanessa Proß¹  Linda Marie Laura Thole¹  Eva Schrezenmeier²  Johannes H. Schulte³  Annette Knabe⁴  Angelika Eggert⁴  Cornelia Eckert⁴  Annika Winkler⁴  Georg Hermsdorf⁴  Hubert Schrezenmeier⁵  Carolin Ludwig⁵ 

[1]Department of General and Visceral Surgery, Charite-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany

[2]Department of Nephrology and Medical Intensive Care, Charite-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany

[3]Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin Institute of Health (BIH) Academy, Clinician Scientist Program Universitätsmedizin Berlin, Berlin, Germany

[4]Department of Pediatric Hematology and Oncology, University Children’s Hospital, Eberhard Karls University Tuebingen, Tuebingen, Germany

[5]Department of Pediatric Oncology and Hematology, Charite-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany

[6]Institute of Transfusion Medicine, Ulm University, Ulm, Germany

[7]Institute for Clinical Transfusion Medicine and Immunogenetics, German Red Cross Blood Transfusion Service Baden-Württemberg – Hessen and University Hospital Ulm, Ulm, Germany

关键词: SARS-CoV2;    mRNA vaccination;    stem cell transplantation;    pediatrics;    cellular immunity;    humoral immunity;    antigen-specific immunity;   

DOI  :  10.3389/fimmu.2023.1239519

 received in 2023-06-13, accepted in 2023-10-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

Stem cell transplant recipients (SCTR) are imperiled to increased risks after SARS-CoV2 infection, supporting the need for effective vaccination strategies for this vulnerable group. With respect to pediatric patients, data on immunogenicity of SARS-CoV2 mRNA-based vaccination is limited. We therefore comprehensively examined specific humoral, B- and T cell responses in a cohort of 2-19 year old SCTR after the second and third vaccine dose. Only after booster vaccination, transplant recipients reached similar levels of vaccine-specific IgG, IgA and neutralizing antibodies against omicron variant as age-matched controls. Although frequencies of SARS-CoV2 specific B cells increased after the third dose, they were still fourfold reduced in patients compared to controls. Overall, the majority of individuals enrolled mounted SARS-CoV2 Spike protein-specific CD4+ T helper cell responses with patients showing significantly higher portions than controls after the third dose. With respect to functionality, however, SCTR were characterized by reduced frequencies of specific interferon gamma producing CD4+ T cells, along with an increase in IL-2 producers. In summary, our data identify distinct quantitative and qualitative impairments within the SARS-CoV2 vaccination specific B- and CD4+ T cell compartments. More importantly, humoral analyses highlight the need for a booster vaccination of SCTR particularly for development of neutralizing antibodies.

【 授权许可】

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Copyright © 2023 Thole, Tóth, Proß, Siegle, Stahl, Hermsdorf, Knabe, Winkler, Schrezenmeier, Ludwig, Eckert, Eggert, Schrezenmeier, Sattler, Schulte and Kotsch