Genome-wide transcription landscape of citric acid producing Aspergillus niger in response to glucose gradient

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Timothy C. Cairns¹  Hongjiang Yang²  Xinrong Ma²  Kaiyue Gao³  Yimou Du³  Peng Du³  Xiaomeng Ni⁴  Jibin Sun⁵  Ping Zheng⁵  Xiaomei Zheng⁵  Wei Zhao⁶  Meiling Chen⁷ 

[1] Chair of Applied and Molecular Microbiology, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany;College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China;College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China;Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China;College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China;Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China;National Technology Innovation Center of Synthetic Biology, Tianjin, China;College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China;Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China;National Technology Innovation Center of Synthetic Biology, Tianjin, China;University of Chinese Academy of Sciences, Beijing, China;Shan Dong Fuyang Biological Technology Co., Ltd., Dezhou, China;Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China;School of Biotechnology, East China University of Science and Technology, Shanghai, China;

关键词: Aspergillus niger;    glucose signaling;    CCR;    citric acid;    carbon utilization;   

DOI  :  10.3389/fbioe.2023.1282314

 received in 2023-08-24, accepted in 2023-10-13,  发布年份 2023

来源: Frontiers

【 摘 要 】

Aspergillus niger is the main industrial workhorse for global citric acid production. This fungus has complex sensing and signaling pathways to respond to environmental nutrient fluctuations. As the preferred primary carbon source, glucose also acts as a critical signal to trigger intracellular bioprocesses. Currently, however, there is still a knowledge gap in systems-level understanding of metabolic and cellular responses to this vital carbon source. In this study, we determined genome-wide transcriptional changes of citric acid-producing Aspergillus niger in response to external glucose gradient. It demonstrated that external glucose fluctuation led to transcriptional reprogramming of many genes encoding proteins involved in fundamental cellular process, including ribosomal biogenesis, carbon transport and catabolism, glucose sensing and signaling. The major glucose catabolism repressor creA maintained a stable expression independent of external glucose, while creB and creD showed significant downregulation and upregulation by the glucose increase. Notably, several high-affinity glucose transporters encoding genes, including mstA, were greatly upregulated when glucose was depleted, while the expression of low-affinity glucose transporter mstC was glucose-independent, which showed clear concordance with their protein levels detected by in situ fluorescence labeling assay. In addition, we also observed that the citric acid exporter cexA was observed to be transcriptionally regulated by glucose availability, which was correlated with extracellular citric acid secretion. These discoveries not only deepen our understanding of the transcriptional regulation of glucose but also shed new light on the adaptive evolutionary mechanism of citric acid production of A. niger.

【 授权许可】

Unknown   
Copyright © 2023 Zheng, Du, Gao, Du, Cairns, Ni, Chen, Zhao, Ma, Yang, Zheng and Sun.