Induction of cytotoxicity of Pelagia noctiluca venom causes reactive oxygen species generation, lipid peroxydation induction and DNA damage in human colon cancer cells

Lipids in Health and Disease

Research

Wiem Zakhama¹  Manel Boussabbeh¹  Yosra Ayed¹  Chayma Bouaziz¹  Salwa Abid¹  Hassen Bacha² 

[1] Laboratory for Research on Biologically Compatible Compounds, Faculty of Dentistry, Rue Avicenne, 5019, Monastir, Tunisia;Laboratory for Research on Biologically Compatible Compounds, Faculty of Dentistry, Rue Avicenne, 5019, Monastir, Tunisia;University of Jendouba, Cité AlFaeiz rue Jamil Boutheina Jendouba, 8100, Tunisia;

关键词: Pelagia noctiluca;    Jellyfish;    Venom;    Cytotoxicity;    Oxidative stress;    DNA fragmentation;   

DOI  :  10.1186/1476-511X-10-232

 received in 2011-10-26, accepted in 2011-12-09,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe long-lasting and abundant blooming of Pelagia noctiluca in Tunisian coastal waters compromises both touristic and fishing activities and causes substantial economic losses. Determining their molecular mode of action is, important in order to limit or prevent the subsequent damages. Thus, the aim of the present study was to investigate the propensity of Pelagia noctiluca venom to cause oxidative damage in HCT 116 cells and its associated genotoxic effects.ResultsOur results indicated an overproduction of ROS, an induction of catalase activity and an increase of MDA generation. We looked for DNA fragmentation by means of the comet assay. Results indicated that venom of Pelagia noctiluca induced DNA fragmentation. SDS-PAGE analysis of Pelagia noctiluca venom revealed at least 15 protein bands of molecular weights ranging from 4 to 120 kDa.ConclusionOxidative damage may be an initiating event and contributes, in part, to the mechanism of toxicity of Pelagia noctiluca venom.

【 授权许可】

Unknown   
© Ayed et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.