A comprehensive and quantitative review of dark fermentative biohydrogen production

Microbial Cell Factories

Review

Christoph Herwig¹  Simon Rittmann¹ 

[1] Institute of Chemical Engineering, Research Area Biochemical Engineering, Gumpendorferstraße 1a, Vienna University of Technology, 1060, Vienna, Austria;

关键词: Hydrogen yield;    Volumetric hydrogen productivity;    Specific hydrogen productivity;    Closed batch;    Batch;    Chemostat culture;    Fed-batch;    Bioprocess;    Result normalization;    Scalable physiological parameters;   

DOI  :  10.1186/1475-2859-11-115

 received in 2012-03-30, accepted in 2012-08-03,  发布年份 2012

来源: Springer

【 摘 要 】

Biohydrogen production (BHP) can be achieved by direct or indirect biophotolysis, photo-fermentation and dark fermentation, whereof only the latter does not require the input of light energy. Our motivation to compile this review was to quantify and comprehensively report strains and process performance of dark fermentative BHP. This review summarizes the work done on pure and defined co-culture dark fermentative BHP since the year 1901. Qualitative growth characteristics and quantitative normalized results of H2 production for more than 2000 conditions are presented in a normalized and therefore comparable format to the scientific community.Statistically based evidence shows that thermophilic strains comprise high substrate conversion efficiency, but mesophilic strains achieve high volumetric productivity. Moreover, microbes of Thermoanaerobacterales (Family III) have to be preferred when aiming to achieve high substrate conversion efficiency in comparison to the families Clostridiaceae and Enterobacteriaceae.The limited number of results available on dark fermentative BHP from fed-batch cultivations indicates the yet underestimated potential of this bioprocessing application. A Design of Experiments strategy should be preferred for efficient bioprocess development and optimization of BHP aiming at improving medium, cultivation conditions and revealing inhibitory effects. This will enable comparing and optimizing strains and processes independent of initial conditions and scale.

【 授权许可】

CC BY   
© Rittmann and Herwig; licensee BioMed Central Ltd. 2012