Photo-biohydrogen production potential of Rhodobacter capsulatus-PK from wheat straw

Biotechnology for Biofuels

Saima Shahzad Mirza¹  Javed Iqbal Qazi²  Quanbao Zhao¹  Shulin Chen¹ 

[1] Biological Systems Engineering, Washington State University, Bioprocessing and Bioproduct Engineering Laboratory, Pullman, WA, USA

[2] Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, 54590 Lahore, Pakistan

关键词: Acetic acid and H₂;    Furfural and H₂;    Hydrogen yield and monomeric sugars;    Cellulose;    Hydrogen yield;    PNSB;   

Others  :  797880
DOI  :  10.1186/1754-6834-6-144

 received in 2013-04-14, accepted in 2013-09-27,  发布年份 2013

【 摘 要 】

Background

Biotechnological exploitation of lignocellulosic biomass is promising for sustainable and environmentally sound energy provision strategy because of the abundant availability of the renewable resources. Wheat straw (WS) comprising of 75-80% cellulose and hemicellulose is one of widely available, inexpensive and renewable lignocellulosic biomass types. The cellulosic and hemicellulose substrate can be hydrolyzed into monomeric sugars by chemical and/or biological methods.

Results

This study examined comparative potential of dilute acid and pre-ammonia pretreated and enzymatically hydrolyzed wheat straw (WS) for hydrogen production by purple non sulfur bacterium Rhodobacter capsulatus-PK. Gas production became noticeable after 14 h of inoculation in WS pretreated with 4% H₂SO₄. The detoxified liquid hydrolyzate (DLH) after overliming attained a production level of 372 mL-H₂/L after 16 h under illumination of 120-150 W/m² at 30 ± 2.0°C. Whereas the non-detoxified acid pretreated hydrolyzate (NDLH) of WS could produce only upto 254 mL-H₂/L after 21 h post inoculation. Evolution of H₂ became observable just after 10 ± 2.0 h of inoculation by employing 48 h age inoculum on the WS pretreated with 30% ammonia, hydrolyzed with cellulase 80 FPU/g and β-glucosidase 220 CbU/ml at 50°C. Upto 712 ml/L of culture was measured with continuous shaking for 24 h. The 47.5% and 64.2% higher hydrogen volume than the DLH and NDLH substrates, respectively appeared as a function of significantly higher monomeric sugar contents of the enzymatically hydrolyzed substrate and lesser/zero amounts of toxic derivatives including pH reducing agents.

Conclusion

Photofermentative hydrogen production from lignocellulosic waste is a feasible approach for eco-friendly sustainable supply of bioenergy in a cost-effective way. Results of this study provide new insight for addressing biotechnological exploitation of abundantly available and low-cost cellulosic substrates.

【 授权许可】

   
2013 Mirza et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

【 参考文献 】

• [1]Wen Hay JX, Wu TY, Teh CY, Jahim JM: Optimized growth of Rhodobacter sphaeroides O. U. 001 using response surface methodology (RSM). J Sci Ind Res 2012, 71:149-154.
• [2]Basak N, Das D: The prospect of purple non sulfur (PNS) photosynthetic bacteria for hydrogen production: the present state of the art. World J Microbial Biotechnol 2007, 23:31-42.
• [3]Keskin T, Hallenbeck PC: Hydrogen production from sugar industry wastes using single-stage photofermentation. Bioresour Technol 2012, 112:131-136.
• [4]Han H, Liu B, Yang H, Shen J: Effect of carbon sources on the photobiological production of hydrogen using Rhodobacter sphaeroides RV. Int J Hydrogen Energy 2012, 37:12167-12174.
• [5]Koku H, Erolu I, Gunduz U, Yucel M, Turker L: Aspect of the metabolism of hydrogen production by Rhodobacter sphaeroides. Int J Hydrogen Energy 2002, 27:1315-1329.
• [6]Han M, Moon SK, Kim Y, Kim Y, Chung B, Choi GW: Bioethanol production from ammonia percolated wheat straw. Biotechnology and Bioprocess Eng 2009, 14:606-611.
• [7]Wyman CE, Dale BE, Elander RT, Holtzapple M, Ladisch MR, Lee YY: Coordinated development of leading biomass pretreatment technologies. Bioresour Technol 2005, 96:1959-1966.
• [8]Taherzadeh MJ, Karimi K: Pretreatment of lignocellulosic wastes to improve ethanol and biogas production: a review. Int J Mol Sci 2008, 9:1621-1651.
• [9]Wyman CE: Biomass ethanol: Technical progress, opportunities, and commercial chanllenges. Ann Rev Energy Environ 1999, 24:189-226.
• [10]Wen Z, Liao W, Chen S: Hydrolysis of animal manure lignocellulosics for reducing sugar production. Bioresour Technol 2004, 91:31.
• [11]Chang V, Holtzapple M: Fundamental factors affecting biomass enzymatic reactivity. Appl Biochem Biotechnol 2000, 84–86:5-37.
• [12]Qi B, Chen X, Shen F, Su Y, Wan Y: Optimization of enzymatic hydrolysis of wheat straw pretreated by alkaline peroxide using response surface methodology. Ind Eng Chem Res 2009, 48:7346-7353.
• [13]Yu X, Zheng Y, Dorgan KM, Chen S: Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid. Bioresour Technol 2011, 102:6134-6140.
• [14]Chang YN, Huang J-C, Lee C-C: Use of response surface methodology to optimize culture medium for production of lovastatin by Monascus rubber. Enzyme Microb Technol 2002, 30:889-894.
• [15]Gosh D, Sobro IF, Hallenbeck PC: Optimization of hydrogen yield from single stage photofermentation of glucose by Rhodobacter capsulatus JP91 using response surface methodology. Bioresour Technol 2012, 123:199-206.
• [16]Gabrielyan L, Torgomyan H, Trchounian A: Growth characteristics and hydrogen production by Rhodobacter sphaeroides using various amino acids as nitrogen sources and their combinations with carbon sources. Int J Hydrogen Energy 2010, 35:12201-12207.
• [17]Remond C, Aubry N, Cronier D, Noel S, Martel F, Roge B, Rakotoarivonina H, Debeire P, Chabbert B: Combination of ammonia and xylanase pretreatment: Impact on enzymatic xylan and cellulose recovery from wheat straw. Bioresour Technol 2010, 101:6712-6717.
• [18]Garcia-Cubero MT, Gonzalez-Benito G, Indacoechea I, Coca M, Bolado S: Effect of ozonolysis pretreatment on enzymatic digestibility of wheat and rye straw. Bioresour Technol 2009, 100:1608-1613.
• [19]Pan C-M, Fan Y-T, Zhao P, Hou H-W: Fermentative hydrogen production by the newly isolated Clostridium beijerinckii Fanp3. Int J Hydrogen Energy 2008, 33:5383-5391.
• [20]Liao W, Wen ZY, Hurley S, Liu Y, Liu CB, Chen SL: Effects of hemicelluloses and lignin on enzymatic hydrolysis of cellulose from dairy manure. Appl Biochem Biotechnol 2005, 124:1017-1030.
• [21]Yang B, Wyman CE: BSA treatment to enhance enzymatic hydrolysis of cellulose in lignin containing substrates. Biotechnol Bioeng 2006, 94:611-617.
• [22]Mohagheghi A, Ruth M, Schell DJ: Conditioning hemicellulose hydrolysates for fermentation: effects of overliming pH on sugar and ethanol yields. Process Biochem 2006, 41:1806-1811.
• [23]Pattra S, Sangyoka S, Boonmee M, Reungsang A: Bio hydrogen production from the fermentation of sugarcane bagasse hydrolysate by Clostridium butyricum. Int J Hydrogen Energy 2008, 33:5256-5265.
• [24]Aguilar R, Ramirez JA, Garrote G, Vazquez M: Kinetic study of the acid hydrolysis of sugarcane bagasse. J Food Eng 2002, 55:309-318.
• [25]Liu Y, Yu P, Song X, Qu Y: Hydrogen production from cellulose by co-culture of Clostridium thermocellum JN4 and thermoanaerobacterium thermosaccharolyticum GD17. Int J Hydrogen Energy 2008, 33:2927-2933.
• [26]De Vrije T, Bakker RR, Budde MAW, Lai MH, Mars AE, Claassen PAM: Efficient hydrogen production from the lignocellulosic energy crop Miscanthus by the extreme thermophilic bacteria Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana. Biotechnology Biofuels 2009, 2:12.
• [27]Kaparaju P, Serrano M, Thomsen AB, Kongjan P, Angelidaki I: Bioethanol, biohydrogen and biogas production from wheat straw in a biorefinery concept. Bioresour Technol 2009, 100:2562-2568.
• [28]Tan H, Yang R, Sun W, Wang S: Peroxide-Acetic acid pretreatment to remove bagasse lignin prior to enzymatic hydrolysis. Ind Eng Chem Res 2010, 49:1473-1479.
• [29]Yue Z, Teater C, Maclellan J, Liu Y, Liao W: Development of a new bioethanol feedstock- Anaerobically digested fiber from confined dairy operations using different digestion configurations. Biomass Bioenergy 2011, 35:1946-1953.
• [30]Anam K, Habibi MS, Harwati UT, Susilaningsih D: Photofermentative hydrogen production using Rhodobium marinum from bagasse and soy sauce wastewater. Int J Hydrogen Energy 2012, 37:15436-15442.
• [31]Zhao Y, Wang Y, Zhu JY, Ragauskas A, Deang Y: Enhanced enzymatic hydrolysis of spruce by alkaline pretreatment at low temperature. Biotech Bioeng 2008, 99:1320-1328.
• [32]Hendriks ATWM, Zeeman G: Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresour Technol 2009, 100:10-18.
• [33]Kumar P, Barret DM, Delwiche MJ, Stroeve P: Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production. Ind Eng Chem Res 2009, 48:3713-3729.
• [34]Hakobyan L, Gabrielyan L, Trchounian A: Yeast extract as an effective nitrogen source stimulating cell growth and enhancing hydrogen photoproduction by Rhodobacter sphaeroides strains from mineral springs. Int J Hydrogen Energy 2012, 37:6519-6526.
• [35]Kern M, Klipp W, Klemme JH: Increased nitrogenase- dependent H2 photoproduction by hup mutants of Rhodospirillum rubrum. Appl Environ Microbiol 1994, 60:1768-1774.
• [36]Shi XY, Yu HQ: Optimization of glutamate concentration and pH for H2 production from volatile fatty acids by Rhodopseudomonas capsulata. Lett Appl Microbiol 2005, 40:401-406.
• [37]Salerno MB, Park W, Zuo Y, Logan BE: Inhibition of biohydrogen production by ammonia. Water Res 2006, 40:1167-1172.
• [38]Abo-Hashesh M, Hallenbeck PC: Microaerobic dark fermentative hydrogen production by the photosynthetic bacterium, Rhodobacter capsulatus JP91. Int J Low-Carbon Technologies 2012, 7:97-103.
• [39]Bashir MJK, Isa MH, Kutty SRM, Awang ZB, Aziz HA, Mohajeri S, Farooqi IH: Landfill leachate treatment by electrochemical oxidation. Waste Manage 2009, 29:2534-2541.
• [40]Basak N, Das D: Photofermentative hydrogen production using purple non sulfur bacteria Rhodobacter sphaeroides O.U.001 in an annular photobioreactor: A case study. Biomass Bioenergy 2009, 33:911-919.
• [41]Gregg DJ, Saddler JN: Factors affecting cellulose hydrolysis and the potential of enzyme recycle to enhance the efficiency of an integrated wood to ethanol process. Biotech. Bioeng 1996, 51:375-383.
• [42]Barbosa MJ, Rocha JMS, Tramper J, Wijffels RH: Acetate as a carbon source for hydrogen production by photosynthetic bacteria. J Biotechnol 2001, 85:25-33.
• [43]Yokoi H, Maeda Y, Hirose J, Hayashi S: Hydrogen production by immobilized cell of Clostridium butyricum on porous glass beads. Biotechnol Tech 1997, 11:431-433.
• [44]Chin HL, Chen ZS, Chou CP: Fed batch operation using Clostridium acetobutylicum suspension culture as biocatalyst for enhancing hydrogen production. Biotechnol Prog 2003, 19:383-388.
• [45]Abo-Hashesh M, Ghosh D, Tourigny A, Taous A, Hallenbeck PC: Single stage photofermentative hydrogen production from glucose: an attractive alternative to two stage photofermentation or co-culture approaches. Int J Hydrogen Energy 2011, 36:13889-13895.
• [46]Hallenbeck PC, Ghosh D, Skonieczny YV: Microbiological and engineering aspects of biohydrogen production. Ind J Microbiol 2009, 49:48-59.
• [47]Jamil Z, Annuar MSM, Ibrahim S, Vikineswary S: Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design. Int J Hydrogen Energy 2009, 7502:7512.
• [48]Golomysova A, Gomelsky M, Ivanov PS: Flux balance analysis of photoheterotrophic growth of purple non sulfur bacteria relevant to biohydrogen production. Int J Hydrogen Energy 2010, 35:12751-12760.
• [49]Kondo T, Arakawa M, Hirai T, Wakayama T, Hara M, Miyaye J: Enhancement of hydrogen production by a photosynthetic bacterium mutant with reduced pigment. J Biosci Bioeng 2002, 93:145-150.
• [50]Biebl H, Pfennig N: Isolation of members of family Rhodosprillaceae. In The prokaryotes. volume 1. Edited by Starr MP, Stolp H, Truper HG, Balows A, Schlegel HG. New York: Springer; 1981:267-273.
• [51]Mirza SMA: Wastewater treatment and hydrogen production potential of locally isolated non sulfur purple bacteria. Lahore: PhD Thesis, University of the Punjab, Department of Zoology; 2012.
• [52]Chen CY, Liu CH, Lo YC, Chang JS: Perspectives on cultivation strategies and photobioreactor designs for photo-fermentative hydrogen production. Bioresour Technol 2011, 102:8484-8492.
• [53]Zhu H, Ueda S, Asada Y, Miyake J: Hydrogen production as a novel process of wastewater treatment-studies on tofu wastewater with entrappedR. sphaeroidesand mutagenesis.Int. J. Hydrogen Energy 2002, 27:1349-1357.
• [54]Eroglu E, Gunduz U, Yucel M, Turker L, Eroglu I: Photobiological hydrogen production from olive mill wastewater as sole substrate sources. Int J Hydrogen Energy 2004, 29:163-171.
• [55]Özgür E, Afsar N, De Vrije T, Yücel M, Gündüz U, Claassen PAM, Eroglu I: Potential use of thermophilic dark fermentation effluents in photofermentative hydrogen production by Rhodobacter capsulatus. J Clean Prod 2010, 18:S23-S28.
• [56]Boran E, Ozgur E, Yucel M, Gunduz U, Eroglu I: Biohydrogen production by Rhodobacter capsulatus in solar tubular photobioreactor on thick juice dark fermenter effluent. J Clean Prod 2012, 31:150-157.
• [57]Fang HHP, Liu H, Zhang T: Phototrophic hydrogen production from acetate and butyrate in wastewater. Int J Hydrogen Energy 2005, 30:785-793.
• [58]Thakur C, Srivastava VC, Mall ID: Electrochemical treatment of a distillery wastewater: Parametric and residue disposal study. Chem Eng J 2009, 148:496-505.
• [59]ASTM: Standard Test Method for Ethanol-Toluene Solubility of Wood. In Annual Book of Wood Standards. Philadelphia, US; 1996.
• [60]Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D: Determination of structural carbohydrates and lignin in Biomass. Golden, CO: Laboratory Analytical Procedure (LAP), National Renewable Energy Laboratory (NREL); 2008.
• [61]Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D: Determination of Sugars, Byproducts, and Degradation Products in Liquid Fraction Process Samples. Golden, CO: Laboratory Analytical Procedure (LAP), National Renewable Energy Laboratory (NREL); 2006.