Iron oxide nanoparticles for neuronal cell applications: uptake study and magnetic manipulations

Journal of Nanobiotechnology

Research

Shlomo Margel¹  Itay Levy¹  Orit Shefi²  Moshe Karni²  Noa Alon²  Koby Baranes²  Michal Marcus² 

[1] Department of Chemistry, Bar Ilan University, Ramat Gan, Israel;Bar Ilan Institute of Nanotechnologies and Advanced Materials, Ramat Gan, Israel;Neuro-engineering lab, Faculty of Engineering, Bar Ilan University, Ramat Gan, Israel;Bar Ilan Institute of Nanotechnologies and Advanced Materials, Ramat Gan, Israel;

关键词: Magnetic nanoparticles;    Magnetic field;    Uptake;    Neuronal cells;    Cell positioning;    Guidance;    Neuronal regeneration;   

DOI  :  10.1186/s12951-016-0190-0

 received in 2016-02-14, accepted in 2016-05-04,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe ability to direct and manipulate neuronal cells has important potential in therapeutics and neural network studies. An emerging approach for remotely guiding cells is by incorporating magnetic nanoparticles (MNPs) into cells and transferring the cells into magnetic sensitive units. Recent developments offer exciting possibilities of magnetic manipulations of MNPs-loaded cells by external magnetic fields. In the present study, we evaluated and characterized uptake properties for optimal loading of cells by MNPs. We examined the interactions between MNPs of different cores and coatings, with primary neurons and neuron-like cells.ResultsWe found that uncoated-maghemite iron oxide nanoparticles maximally interact and penetrate into cells with no cytotoxic effect. We observed that the cellular uptake of the MNPs depends on the time of incubation and the concentration of nanoparticles in the medium. The morphology patterns of the neuronal cells were not affected by MNPs uptake and neurons remained electrically active. We theoretically modeled magnetic fluxes and demonstrated experimentally the response of MNP-loaded cells to the magnetic fields affecting cell motility. Furthermore, we successfully directed neurite growth orientation along regeneration.ConclusionsApplying mechanical forces via magnetic mediators is a useful approach for biomedical applications. We have examined several types of MNPs and studied the uptake behavior optimized for magnetic neuronal manipulations.

【 授权许可】

CC BY   
© The Author(s). 2016