In vitro effects of platelet-rich gel supernatants on histology and chondrocyte apoptosis scores, hyaluronan release and gene expression of equine cartilage explants challenged with lipopolysaccharide

BMC Veterinary Research

Research Article

Jorge U. Carmona¹  Diana L. Ríos¹  Catalina López¹  María E. Álvarez²  Mabel E. Bohórquez³  Jorge E. Pérez⁴ 

[1] Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia;Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia;Grupo de investigación Biosalud, Departamento de Ciencias Básicas para la Salud, Universidad de Caldas, Manizales, Colombia;Grupo de Investigación en Citogenética, Filogenia y Evolución de Poblaciones, Universidad del Tolima, Ibagué, Colombia;Grupo de investigación Biosalud, Departamento de Ciencias Básicas para la Salud, Universidad de Caldas, Manizales, Colombia;

关键词: Platelet-rich plasma;    Cartilage explants;    Chondrocyte apoptosis;    Catabolic/anabolic gene expression;    Growth factors;   

DOI  :  10.1186/s12917-016-0759-8

 received in 2015-10-14, accepted in 2016-06-23,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundPlatelet-rich plasma (PRP) preparations are a common treatment in equine osteoarthritis (OA). However, there are controversies regarding the ideal concentration of platelets and leukocytes in these biological substances necessary to induce an adequate anti-inflammatory and anabolic response in articular cartilage. The aims were to study the influence of leukocyte- and platelet-rich gel (L-PRG) and pure platelet-rich gel (P-PRG) supernatants on the histological changes of cartilage, the degree of chondrocyte apoptosis, the production of hyaluronan (HA) and the gene expression of nuclear factor kappa beta (NFkβ), matrix metalloproteinase 13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4), collagen type I alpha 1 (COL1A1), collagen type II alpha 1 (COL2A1) and cartilage oligomeric matrix protein (COMP) in normal cartilage explants (CEs) challenged with lipopolysaccharide (LPS).ResultsOverall, 25 % L-PRG supernatant (followed in order of importance by, 50 % P-PRG, 25 % P-PRG and 50 % L-PRG) represented the substance with the most important anti-inflammatory and anabolic effect. 25 % P-PRG supernatant presented important anabolic effects, but it induced a more severe chondrocyte apoptosis than the other evaluated substances.Conclusions25 % L-PRG supernatant presented the best therapeutic profile. Our results demonstrate that the biological variability of PRP preparations makes their application rather challenging. Additional in vivo research is necessary to know the effect of PRP preparations at different concentrations.

【 授权许可】

CC BY   
© The Author(s). 2016