Modeling the transboundary risk of feed ingredients contaminated with porcine epidemic diarrhea virus

BMC Veterinary Research

Research Article

Travis Clement¹  Aaron Singrey¹  Jane Christopher-Hennings¹  Eric Nelson¹  Gilbert Patterson²  Cassandra Jones³  Roger Cochrane³  Casey Neill⁴  Scott Dee⁴  Gordon Spronk⁴ 

[1] Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, SD, USA;Center for Animal Health and Food Safety, University of Minnesota, St. Paul, MN, USA;Department of Grain Science, Kansas State University, Manhattan, KS, USA;Pipestone Applied Research, Pipestone Veterinary Services, 1300 Box, 188 Hwy 75 S, 56164, Pipestone, MN, USA;

关键词: Transboundary;    Porcine;    Epidemic;    Diarrhea;    Virus;    Antimicrobial;    Ingredient;    Lysine;    Soybean meal;    Choline;   

DOI  :  10.1186/s12917-016-0674-z

 received in 2015-10-26, accepted in 2016-03-04,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThis study describes a model developed to evaluate the transboundary risk of PEDV-contaminated swine feed ingredients and the effect of two mitigation strategies during a simulated transport event from China to the US.ResultsIngredients imported to the USA from China, including organic & conventional soybeans and meal, lysine hydrochloride, D-L methionine, tryptophan, Vitamins A, D & E, choline, carriers (rice hulls, corn cobs) and feed grade tetracycline, were inoculated with PEDV. Control ingredients, and treatments (ingredients plus a liquid antimicrobial (SalCURB, Kemin Industries (LA) or a 2 % custom medium chain fatty acid blend (MCFA)) were tested. The model ran for 37 days, simulating transport of cargo from Beijing, China to Des Moines, IA, US from December 23, 2012 to January 28, 2013. To mimic conditions on land and sea, historical temperature and percent relative humidity (% RH) data were programmed into an environmental chamber which stored all containers. To evaluate PEDV viability over time, ingredients were organized into 1 of 4 batches of samples, each batch representing a specific segment of transport. Batch 1 (segment 1) simulated transport of contaminated ingredients from manufacturing plants in Beijing (day 1 post-contamination (PC)). Batch 2 (segments 1 and 2) simulated manufacturing and delivery to Shanghai, including time in Anquing terminal awaiting shipment (days 1–8 PC). Batch 3 (segments 1, 2 and 3) represented time in China, the crossing of the Pacific and entry to the US at the San Francisco, CA terminal (day 1–27 PC). Batch 4 (segments 1–4) represented the previous events, including transport to Des Moines, IA (days 1–37 PC). Across control (non-treated) ingredients, viable PEDV was detected in soybean meal (organic and conventional), Vitamin D, lysine hydrochloride and choline chloride. In contrast, viable PEDV was not detected in any samples treated with LA or MCFA.ConclusionsThese results demonstrate the ability of PEDV to survive in a subset of feed ingredients using a model simulating shipment from China to the US. This is proof of concept suggesting that contaminated feed ingredients could serve as transboundary risk factors for PEDV, along with the identification of effective mitigation options.

【 授权许可】

CC BY   
© Dee et al. 2016