Dihydrocapsaicin down-regulates apoM expression through inhibiting Foxa2 expression and enhancing LXRα expression in HepG2 cells

Lipids in Health and Disease

Research

Xin Ma¹  Shu-Fen Li²  Qian Wang²  Yan-Chao Wang²  Lei Zheng²  Ya-Rong Hu²  Jia-Yi Zhao²  Ji-Juan Gao²  Yan-Wei Hu²  Shao-Guo Wu²  Yan-Hua Sha² 

[1] Department of Anesthesiology, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, Guangdong, China;Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, Guangdong, China;

关键词: DHC;    ApoM;    Foxa2;    LXRα;   

DOI  :  10.1186/1476-511X-13-50

 received in 2013-12-12, accepted in 2014-03-13,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundApolipoprotein M (apoM), as a novel apolipoprotein which is mainly expressed in liver and kidney tissues, is associated with development and progression of atherosclerosis and diabetes. Our group have recently shown that Dihydrocapsaicin(DHC)can significantly decrease atherosclerotic plaque formation in apoE−/− mice. However, the effect and possible mechanism of DHC on apoM expression remain unclear.MethodsHepG2 cells were treated with 0 μM, 25 μM, 50 μM and 100 μM DHC for 24 h or were treated with 100 μM DHC for 0, 6, 12, and 24 h, respectively. The mRNA levels and protein levels were measured by real-time quantitative PCR and western blot analysis, respectively.ResultsWe found that DHC markedly decreased expression of apoM at both mRNA and protein level in HepG2 cells in a dose-dependent and time-dependent manner. Expression of Foxa2 was decreased while expression of LXRα was increased by DHC treatment in HepG2 cells. In addittion, overexpression of Foxa2 markedly compensated the inhibition effect induced by DHC on apoM expression. LXRα small interfering RNA significantly abolished the inhibition effect which induced by DHC on apoM expression. The liver of C57BL/6 mice treated with DHC had significantly lower expression of apoM. Furthermore, the liver had lower expression of Foxa2 while had higher expression of LXRα.ConclusionsDHC could down-regulate apoM expression through inhibiting Foxa2 expression and enhancing LXRα expression in HepG2 cells.

【 授权许可】

Unknown   
© Zhao et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.