Ceramide‐induced translocation of protein kinase C ζ in primary cultures of astrocytes

FEBS Letters

Galve-Roperh, Ismael¹  Dı́az-Laviada, Inés²  Haro, Amador¹ 

[1] Department of Biochemistry and Molecular Biology I, Faculty of Biology, Complutense University, 28040 Madrid, Spain;Department of Biochemistry and Molecular Biology, Faculty of Medicine, Alcalá de Henares University, 28871 Alcalá de Henares, Madrid, Spain

关键词: Protein kinase C ζ;    Ceramide;    Sphingomyelin;    Astrocyte;    C2-ceramide;    N-acetylsphingosine;    C8-ceramide;    N-octanoylsphingosine;    DHC;    dihydro-N-acetylsphingosine;    IL;    interleukin;    MAPK;    mitogen-activated protein kinase;    NGF;    nerve growth factor;    PC-PLC;    phosphatidylcholine phospholipase C;    PKC;    protein kinase C;    SM;    sphingomyelin;    TNF;    tumor necrosis factor;   

DOI  :  10.1016/S0014-5793(97)00985-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The present research was undertaken to study the possible involvement of the atypical protein kinase C (PKC) ζ in ceramide signal transduction in primary cultures of rat astrocytes. As shown by Western blot analysis, translocation of immunoreactive PKCζ to the particulate fraction occurred upon exposure of astrocytes to cell-permeable ceramide analogs or to exogenous sphingomyelinase. The particulate fraction may correspond to a perinuclear area, as indicated by immunocytochemical techniques. Furthermore, treatment of cells with N-octanoylsphingosine led to an increased phosphorylation of PKCζ. Results thus show that stimulation of PKCζ may be one of the intracellular events triggered by activation of the sphingomyelin pathway.

【 授权许可】

Unknown