Effect of β-alanine treatment on mitochondrial taurine level and 5-taurinomethyluridine content

Journal of Biomedical Science

Review

Takashi Ito¹  Junichi Azuma¹  Mahmood Mozaffari²  Chian Ju Jong³  Stephen Schaffer³ 

[1] Clinical Evaluation of Medicines and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 565-0871, Osaka, Japan;Department of Oral Biology, Medical College of Georgia, School of Dentistry, Augusta, Georgia, USA;Department of Pharmacology, University of South Alabama, College of Medicine, 36688, Mobile, Alabama, USA;

关键词: Taurine;    Taurine Level;    Taurine Transport;    Mitochondrial Protein Synthesis;    Taurine Content;   

DOI  :  10.1186/1423-0127-17-S1-S25

来源: Springer

【 摘 要 】

BackgroundThe β-amino acid, taurine, is a nutritional requirement in some species. In these species, the depletion of intracellular stores of taurine leads to the development of severe organ dysfunction. The basis underlying these defects is poorly understood, although there is some suggestion that oxidative stress may contribute to the abnormalities. Recent studies indicate that taurine is required for normal mitochondrial protein synthesis and normal electron transport chain activity; it is known that defects in these events can lead to severe mitochondrial oxidative stress. The present study examines the effect of taurine deficiency on the first step of mitochondrial protein synthesis regulation by taurine, namely, the formation of taurinomethyluridine containing tRNA.MethodsIsolated rat cardiomyocytes were rendered taurine deficient by incubation with medium containing the taurine transport inhibitor, β-alanine. The time course of cellular and mitochondrial taurine depletion was measured. The primer extension method was employed to evaluate the effect of β-alanine treatment on taurinomethyluridine content of tRNALeu. The protein levels of ND6 were also determined by Western blot analysis.Resultsβ-alanine caused a time-dependent decrease in cellular taurine content, which were reduced in half after 48 hrs of incubation. The amount of taurine in the mitochondria was considerably less than that in the cytosol and was unaffected by β-alanine treatment. Approximately 70% of the tRNALeu in the untreated cell lacked taurinomethyluridine and these levels were unchanged following β-alanine treatment. Protein content of ND6, however, was significantly reduced after 48 hours incubation with β-alanine.ConclusionsThe taurine levels of the cytosol and the mitochondria are not directly coupled. The β-alanine-mediated reduction in taurine levels is too small to affect taurinomethyluridine levels. Nonetheless, it interferes with mitochondrial protein synthesis, as exemplified by a decrease in ND6 protein content. Thus, β-alanine does not cause alterations in mitochondrial protein synthesis through the lowering of taurine levels.

【 授权许可】

CC BY   
© Schaffer et al; licensee BioMed Central Ltd. 2010