Effects of zinc ex vivo on taurine uptake in goldfish retinal cells

Journal of Biomedical Science

Review

Sonia Nusetti¹  Lucimey Lima¹  Mary Urbina¹ 

[1] Laboratorio de Neuroquímica, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela;

关键词: Zinc;    Taurine;    Trophic Effect;    Taurine Transport;    Free Zinc;   

DOI  :  10.1186/1423-0127-17-S1-S13

来源: Springer

【 摘 要 】

BackgroundTaurine and zinc exert neurotrophic effects in the central nervous system. Current studies demonstrate that Na+/Cl- dependent neurotransmitter transporters, similar to that of taurine, are modulated by micromolar concentrations of zinc. This study examined the effect of zinc sulfate ex vivo on [3H]taurine transport in goldfish retina.MethodsIsolated cells were incubated in Ringer with zinc (0.1–100 µM). Taurine transport was done with 50 nM [3H]taurine or by isotopic dilution with taurine (0.001–1 mM) and 50 nM [3H]taurine.ResultsZinc reduced the capacity of taurine transport without changes in affinity, and caused a noncompetitive inhibition of high affinity taurine transport, with an EC50= 0.072 µM. The mechanism by which zinc affects taurine transport is unknown at the present.ConclusionsThere may be a binding site of zinc in the transporter that affects union or translocation of taurine, or possibly the formation of taurine-zinc complexes, rather than free zinc, could affect the operation of the transporter.

【 授权许可】

CC BY   
© Lima et al; licensee BioMed Central Ltd. 2010