Discrimination of cirrhotic nodules, dysplastic lesions and hepatocellular carcinoma by their vibrational signature

Journal of Translational Medicine

Research

Chengyuan Peng¹  François Le Naour¹  Slávka Kaščáková¹  Franck Chiappini¹  Didier Samuel²  Catherine Guettier³  Natalia Olaya⁴  Christophe Sandt⁵  Ibraheem Yousef⁵  Paul Dumas⁵ 

[1] Inserm, Unité 1193, 94800, Villejuif, France;Univ Paris-Sud, UMR-S1193, 94800, Villejuif, France;Inserm, Unité 1193, 94800, Villejuif, France;Univ Paris-Sud, UMR-S1193, 94800, Villejuif, France;Centre Hépato-Biliaire, AP-HP Hôpital Paul Brousse, 94800, Villejuif, France;Inserm, Unité 1193, 94800, Villejuif, France;Univ Paris-Sud, UMR-S1193, 94800, Villejuif, France;Service d’Anatomopathologie, AP-HP Hôpital Bicêtre, 94275, Le Kremlin-Bicêtre, France;Instituto Nacional de Cancerologia, Bogota, Colombia;SOLEIL Synchrotron, 91192, Gif sur Yvette, France;

关键词: Diagnosis;    Cirrhosis;    Dysplastic nodules;    Hepatocellular carcinoma;    Infrared microspectroscopy;    Synchrotron;   

DOI  :  10.1186/s12967-016-0763-6

 received in 2015-09-25, accepted in 2016-01-02,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundHepatocarcinogenesis is a multistep process characterized in patients with chronic liver diseases by a spectrum of hepatic nodules that mark the progression from regenerative nodules to dysplastic lesions followed by hepatocellular carcinoma (HCC). The differential diagnosis between precancerous dysplastic nodules and early HCC still represents a challenge for both radiologists and pathologists. We addressed the potential of Fourier transform-infrared (FTIR) microspectroscopy for grading cirrhotic nodules on frozen tissue sections.MethodsThe study was focused on 39 surgical specimens including normal livers (n = 11), dysplastic nodules (n = 6), early HCC (n = 1), progressed HCC on alcoholic cirrhosis (n = 10) or hepatitis C virus cirrhosis (n = 11). The use of the bright infrared source emitted by the synchrotron radiation allowed investigating the biochemical composition at the cellular level. Chemical mapping on whole tissue sections was further performed using a FTIR microscope equipped with a laboratory-based infrared source. The variance was addressed by principal component analysis.ResultsProfound alterations of the biochemical composition of the pathological liver were demonstrated by FTIR microspectroscopy. Indeed, dramatic changes were observed in lipids, proteins and sugars highlighting the metabolic reprogramming in carcinogenesis. Quantifiable spectral markers were characterized by calculating ratios of areas under specific bands along the infrared spectrum. These markers allowed the discrimination of cirrhotic nodules, dysplastic lesions and HCC. Finally, the spectral markers can be measured using a laboratory FTIR microscope that may be easily implemented at the hospital.ConclusionMetabolic reprogramming in liver carcinogenesis can constitute a signature easily detectable using FTIR microspectroscopy for the diagnosis of precancerous and cancerous lesions.

【 授权许可】

CC BY   
© Peng et al. 2016