Aspirin inhibits rotavirus replication and alters rat gut microbial composition

Virology Journal

Research

Chang Cheng Liu¹  Yong Gang Li¹  Yang Liu¹  Mei Ling Yu¹  Meng Qi Liu¹  Wei Zhao¹  Xue Jiao Jia¹  ZhouPing Li² 

[1] College of Basic Medical Sciences, Jinzhou Medical University, Jinzhou, China;The first affiliated hospital of Jinzhou Medical University, Jinzhou, China;

关键词: Rotavirus;    Aspirin;    Virus replication;    Gut microbiota;    16S rRNA gene sequencing;    Rat model;   

DOI  :  10.1186/s12985-023-02199-5

 received in 2023-08-07, accepted in 2023-10-02,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundAspirin is widely used to treat various clinical symptoms. Evidence suggests that aspirin has antiviral properties, but little is known about its specific effect against rotavirus.MethodsMA104, Caco-2, and CV-1 cells were infected with rotavirus, and aspirin was added after 12 h. Viral mRNA and titer levels were measured by qRT-PCR and immunofluorescence assays. For in vivo validation, forty specific-pathogen-free SD rats were randomly divided into oral aspirin (ASP) groups and control (NC) groups. 16 S rRNA gene sequencing was performed to identify gut microbiota. After 6 months of continuous ASP/NC administration, the rats were infected with rotavirus. Fecal samples were collected over a 30-day time course, and viral levels were quantified. Proinflammatory cytokines/chemokine levels were measured by ELISA.ResultsAspirin inhibited rotavirus infection in cell lines and in rats. The effects of aspirin on viral replication were associated with the alteration of gut microbiota composition by aspirin, including increased abundance of Firmicutes and decreased abundance of Bacteroidetes after aspirin treatment. Mechanistically, aspirin reduced IL-2 and IL-10 levels, and increased IRF-1 and COX-2 levels. Aspirin blocked rotavirus replication in vitro and in vivo, which might be related to effects on IRF-1, COX-2, chemokines, and gut microbial composition.ConclusionThese results indicate that long-term oral aspirin administration reduces rotavirus infection. Intestinal virus infection may be suppressed in elderly patients who take aspirin for a long time. The change of their Gut microbiota may lead to functional disorder of the intestinal tract, which may provide some reference for clinical adjuvant probiotics treatment.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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