Small GTP-binding protein PdRanBP regulates vascular tissue development in poplar

BMC Genetics

Research Article

Shaofeng Li¹  Xue Liu¹  Zanmin Hu²  Jianhui Zhang³  Bingyu Zhang⁴  Changjun Ding⁴  Qinjun Huang⁴  Mengzhu Lu⁴  Xiaohua Su⁴ 

[1] Experimental Center of Forestry in North China, Chinese Academy of Forestry, 100023, Beijing, People’s Republic of China;Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People’s Republic of China;Plants for Human Health Institute, Department of Horticultural Science, North Carolina State University, 600 Laureate Way, 28081, Kannapolis, North Carolina, USA;Biomarker Technologies Corporation, 101300, Beijing, People’s Republic of China;State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, 100091, Beijing, People’s Republic of China;

关键词: Molecular cloning;    Functional analysis;    PdRanBP;    Populus deltoides;    Vascular tissue;   

DOI  :  10.1186/s12863-016-0403-4

 received in 2016-03-16, accepted in 2016-06-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundPrevious research has demonstrated that ectopic expression of Ran-binding protein (RanBP) in Arabidopsis results in more axillary buds and reduced apical dominance compared to WT plants. However, the function of RanBP in poplar, which has very typical secondary growth, remains unclear. Here, the Populus deltoides (Marsh.) RanBP gene (PdRanBP) was isolated and functionally characterized by ectopic expression in a hybrid poplar (P. davidiana Dode × P. bolleana Lauche).ResultsPdRanBP was predominantly expressed in leaf buds and tissues undergoing secondary wall expansion, including immature xylem and immature phloem in the stem. Overexpression of PdRanBP in poplar increased the number of sylleptic branches and the proportion of cells in the G2 phase of the cell cycle, retarded plant growth, consistently decreased the size of the secondary xylem and secondary phloem zones, and reduced the expression levels of cell wall biosynthesis genes. The downregulation of PdRanBP facilitated secondary wall expansion and increased stem height, the sizes of the xylem and phloem zones, and the expression levels of cell wall biosynthesis genes.ConclusionsThese results suggest that PdRanBP influences the apical and radial growth of poplar trees and that PdRanBP may regulate cell division during cell cycle progression. Taken together, our results demonstrated that PdRanBP is a nuclear, vascular tissue development-associated protein in P. deltoides.

【 授权许可】

CC BY   
© The Author(s). 2016