MiR-204 regulates cardiomyocyte autophagy induced by ischemia-reperfusion through LC3-II

Journal of Biomedical Science

Research

Bin He¹  Jian Xiao²  Yufeng Zhang²  Bo Kang²  Zhinong Wang²  Xin Ni³  Xiaoyan Zhu³ 

[1] Department of Anesthesiology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Kongjiang road 1665#, Shanghai, China, 200092;Department of Cardiothoracic Surgery, Changzheng Hospital, the second military medical university, Fengyang road 415#, 200003, shanghai, China;Department of Physiology, the second military medical university, Xiangyin road 800#, 200433, Shanghai, China;

关键词: Left Anterior Descend;    Evans Blue;    Animal Research Ethic Committee;    Infarct Size Measurement;    Computerize Planimetry;   

DOI  :  10.1186/1423-0127-18-35

 received in 2011-01-16, accepted in 2011-06-01,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundAutophagy plays a significant role in myocardial ischemia-reperfusion (IR) injury. So it is important to inhibit autophagy to protect cardiomyocytes besides anti-apoptosis. MiRNA has been demonstrated to protect cardiomyocytes against apoptosis during IR, while whether it has anti-autophagy effect has not been known. The aim of this study was to investigate whether miR-204 regulated autophagy by regulating LC3-II protein, which is the marker of autophagosome during myocardial IR injury.MethodsAdult SD rats were randomized to Control and IR groups. IR group was treated with 30 min ischemia by ligating the left anterior descending coronary artery, followed by 2 h reperfusion by loosing the ligation. The expression of miR-204 was measured by RT-PCR, and LC3 protein was measured by western-blot.ResultsWe found that IR induced cardiomyocytes autophagy, together with down-regulation of miR-204 and up-regulation of LC3-II protein. And, we have found that LC3-II protein was regulated by miR-204, using the method of transferring miR-204 mimic or AMO-204 into the cardiomyocytes, before.ConclusionsThese studies provided evidence that miR-204 played an important role in regulating autophagy through LC3-IIprotein during IR.

【 授权许可】

Unknown   
© Xiao et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.