CMR Assessment of endothelial damage and angiogenesis in porcine coronary arteries using gadofosveset

Journal of Cardiovascular Magnetic Resonance

Research

Erling Falk¹  Troels Thim¹  Won Y Kim²  William P Paaske³  Steen F Pedersen⁴  Samuel A Thrysøe⁵  Steffen Ringgaard⁵ 

[1] Dept. of Cardiology, Aarhus University Hospital Skejby, Brendstrupsgaardsvej 100, 8200, Aarhus N, Denmark;Dept. of Cardiology, Aarhus University Hospital Skejby, Brendstrupsgaardsvej 100, 8200, Aarhus N, Denmark;MR-center, Aarhus University Hospital Skejby, Brendstrupsgaardsvej 100, 8200, Aarhus N, Denmark;Dept. of Cardiothoracic and Vascular Surgery T, Aarhus University Hospital Skejby, Brendstrupsgaardsvej 100, 8200, Aarhus N, Denmark;Dept. of Cardiothoracic and Vascular Surgery T, Aarhus University Hospital Skejby, Brendstrupsgaardsvej 100, 8200, Aarhus N, Denmark;MR-center, Aarhus University Hospital Skejby, Brendstrupsgaardsvej 100, 8200, Aarhus N, Denmark;MR-center, Aarhus University Hospital Skejby, Brendstrupsgaardsvej 100, 8200, Aarhus N, Denmark;

关键词: Cardiovascular Magnetic Resonance;    Left Anterior Descend;    Evans Blue;    Endothelial Damage;    Cardiovascular Magnetic Resonance Image;   

DOI  :  10.1186/1532-429X-13-10

 received in 2010-08-30, accepted in 2011-01-26,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundEndothelial damage and angiogenesis are essential for atherosclerotic plaque development and destabilization. We sought to examine whether contrast enhanced cardiovascular magnetic resonance (CMR) using gadofosveset could show endothelial damage and neovessel formation in balloon injured porcine coronary arteries.Methods and ResultsData were obtained from seven pigs that all underwent balloon injury of the left anterior descending coronary artery (LAD) to induce endothelial damage and angiogenesis. Between one - 12 days (average four) after balloon injury, in vivo and ex vivo T1-weighted coronary CMR was performed after intravenous injection of gadofosveset. Post contrast, CMR showed contrast enhancement of the coronary arteries with a selective and time-dependent average expansion of the injured LAD segment area of 45% (p = 0.04; CI95 = [15%-75%]), indicating local extravasation of gadofosveset. Vascular and perivascular extravasation of albumin (marker of endothelial leakiness) and gadofosveset was demonstrated with agreement between Evans blue staining and ex vivo CMR contrast enhancement (p = 0.026). Coronary MRI contrast enhancement and local microvessel density determined by microscopic examination correlated (ρ = 0.82, p < 0.001).ConclusionContrast enhanced coronary CMR with gadofosveset can detect experimentally induced endothelial damage and angiogenesis in the porcine coronary artery wall.

【 授权许可】

CC BY   
© Pedersen et al; licensee BioMed Central Ltd. 2011