Detection of circulating natural antibodies to inflammatory cytokines in type-2 diabetes and clinical significance

Journal of Inflammation

Research

Cailing Qiu¹  Xiangyun Chen¹  Jun Wei²  Qingyong Meng³  Weiyi Cai³  Hongyu Zhang⁴  Xuan Zhang⁴ 

[1] Dalang Hospital of Dongguan, 523000, Dongguan, China;Division of Health Research, University of the Highlands & Islands, Centre for Health Science, Perth Road, IV2 3JH, Inverness, UK;Laboratory for Nursing Science & Institute of Laboratory Medicine, Guangdong Medical University, No.1 Xincheng Road, 523808, Dongguan, China;The Second Hospital, Jilin University, 130041, Changchun, China;

关键词: Natural antibodies;    IgG antibody;    Inflammatory cytokines;    Type-2 diabetes;    ELISA;   

DOI  :  10.1186/s12950-017-0171-6

 received in 2017-07-26, accepted in 2017-10-28,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundInflammatory cytokines have been demonstrated to be involved in developing insulin resistance and type-2 diabetes (T2D). Natural antibodies in the circulation have protective effects on common diseases in humans. The present study was thus designed to test the hypothesis that natural antibodies against inflammatory cytokines could be associated with T2D.MethodsAn enzyme-linked immunosorbent assay (ELISA) was developed in-house to detect plasma IgG against peptide antigens derived from interleukin 1α (IL1α), IL1β, IL6, IL8 and tumor necrosis factor-α (TNF-α) in 200 patients with T2D and 220 control subjects.ResultsBinary regression showed that compared with control subjects, T2D patients had a decreased level of plasma anti-IL6 IgG (adjusted r2=0.034, p=0.0001), anti-IL8 IgG (adjusted r2=0.021, p=0.002) and anti-TNF-α IgG (adjusted r2=0.017, p=0.003). Female patients mainly contributed to decreased levels of anti-IL6 IgG (adjusted r2=0.065, p=0.0008) and anti-IL8 IgG (adjusted r2=0.056, p=0.003), while male patients mainly contributed to decreased anti-TNF-α IgG levels (adjusted r2=0.024, p=0.005). ROC curve analysis revealed a sensitivity of 16.5% against specificity of 95.5% for anti-IL6 IgG assay and a sensitivity of 19.5% against specificity of 95.9% for anti-IL8 IgG assay. Glycated hemoglobin levels measured after 6-month glucose-lowering treatment appeared to be inversely correlated with plasma anti-IL1α IgG (r=-0.477, df=17, p=0.039) and anti-IL6 IgG (r=-0.519, df=17, p=0.023) although such correlation failed to survive the Bonferroni correction.ConclusionsDeficiency of natural IgG against inflammatory cytokines is likely to be a risk factor for T2D development and detection of such antibodies may be useful for personalized treatment of the disease.

【 授权许可】

CC BY   
© The Author(s). 2017