A next generation sequencing based approach to identify extracellular vesicle mediated mRNA transfers between cells

BMC Genomics

Methodology Article

Kalyani V. Guntur¹  Vivek K. Vishnudas¹  Stephane Gesta¹  Viatcheslav R. Akmaev¹  Jyoti Ranjan¹  Niven R. Narain¹  Rangaprasad Sarangarajan¹  Robert Sebra²  Jun Zhu²  Jialiang Yang²  Kimaada Allette²  Francesca Petralia²  Bin Zhang²  Jacob Hagen²  Zhidong Tu²  Milind Mahajan²  Sander Houten²  Eric E. Schadt²  Andrew Kasarskis²  Sarah Schuyler²  Carmen A. Argmann² 

[1] BERG, LLC, 01701, Framingham, MA, USA;Institute of Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, 10029, New York, NY, USA;Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, 10029, New York, NY, USA;

关键词: Extracellular RNA (exRNA);    Exosome;    Cell-to-cell communication;    RNA-seq;    Macrophages;    Adipocytes;    Bayesian method;   

DOI  :  10.1186/s12864-017-4359-1

 received in 2017-05-14, accepted in 2017-11-29,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundExosomes and other extracellular vesicles (EVs) have emerged as an important mechanism of cell-to-cell communication. However, previous studies either did not fully resolve what genetic materials were shuttled by exosomes or only focused on a specific set of miRNAs and mRNAs. A more systematic method is required to identify the genetic materials that are potentially transferred during cell-to-cell communication through EVs in an unbiased manner.ResultsIn this work, we present a novel next generation of sequencing (NGS) based approach to identify EV mediated mRNA exchanges between co-cultured adipocyte and macrophage cells. We performed molecular and genomic profiling and jointly considered data from RNA sequencing (RNA-seq) and genotyping to track the “sequence varying mRNAs” transferred between cells. We identified 8 mRNAs being transferred from macrophages to adipocytes and 21 mRNAs being transferred in the opposite direction. These mRNAs represented biological functions including extracellular matrix, cell adhesion, glycoprotein, and signal peptides.ConclusionsOur study sheds new light on EV mediated RNA communications between adipocyte and macrophage cells, which may play a significant role in developing insulin resistance in diabetic patients. This work establishes a new method that is applicable to examining genetic material exchanges in many cellular systems and has the potential to be extended to in vivo studies as well.

【 授权许可】

CC BY   
© The Author(s). 2017

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