Expression and role of oncogenic miRNA-224 in esophageal squamous cell carcinoma

BMC Cancer

Research Article

Shuo Li¹  Hong Zhu¹  Bin Xiao¹  Xiaoyan He²  Ming Li³  Zhimei Zhang⁴  Lihua Ren⁵  Ruihua Shi⁶ 

[1] Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, China;Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, China;Department of Gastroenterology, Dongyang People’s Hospital, 60 Wuningxi Road, Jinhua, China;Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, China;Department of Gastroenterology, Friendship Hospital of Yangzhou, 440 Siwangting Road, Yangzhou, China;Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, China;Department of Gastroenterology, The First People’s Hospital of Lianyungang, 182 Tongguanbei Road, Lianyungang, China;Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, China;Department of Gastroenterology, Zhangjiagang First People’s Hospital, 68 Jiyangxi Road, Suzhou, China;Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, China;Department of Gastroenterology, Zhongda Hospital, Southeast University, 87 Dingjiaqiao Road, Nanjing, China;

关键词: Esophageal Cancer;    Esophageal Squamous Cell Carcinoma;    Esophageal Squamous Cell Carcinoma Cell;    Esophageal Squamous Cell Carcinoma Patient;    Esophageal Squamous Cell Carcinoma Tissue;   

DOI  :  10.1186/s12885-015-1581-6

 received in 2014-10-17, accepted in 2015-07-27,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAberrant expression of miR-224 is associated with tumor development and progression. This study investigated the role of miR-224 in esophageal squamous cell carcinoma (ESCC) ex vivo and in vitro.MethodsA total of 103 esophageal intraepithelial neoplasia, ESCC tissue specimens, and their matched distant normal tissues were collected to test miR-224 expression using qRT-PCR analysis. Western blot was used to quantify the level of PH domain leucine-rich repeat protein phosphatase 1 (PHLPP1) and PHLPP2 in ESCC tissues. Cell viability, apoptosis, invasion, and colony formation assays were used to assess the altered phenotypes of esophageal cancer cell lines after miR-224 expression or inhibition. A luciferase reporter assay was used to confirm miR-224 binding to PHLPP1 and PHLPP2 mRNA.ResultsmiR-224 was significantly overexpressed in esophageal intraepithelial neoplasia and ESCC tissues, while the expression of PHLPP1 and PHLPP2 proteins, the target genes of miR-224, was downregulated in ESCC tissues. miR-224 expression was associated with advanced clinical TNM stage, pathologic grade, and the level of PHLPP1 and PHLPP2 proteins in ESCC tissues. Ectopic overexpression of miR-224 promoted proliferation, migration, and invasion, but suppressed apoptosis of ESCC cells. miR-224 was able to bind to the 3′ untranslated region (3′-UTR) of PHLPP1 and PHLPP2 mRNA to suppress their expression.ConclusionsThe current study demonstrated that miR-224 acts as an oncogenic miRNA in ESCC, possibly by targeting PHLPP1 and PHLPP2.

【 授权许可】

Unknown   
© He et al. 2015. This article is published under license to BioMed Central Ltd. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.