PRAF3 induces apoptosis and inhibits migration and invasion in human esophageal squamous cell carcinoma

BMC Cancer

Research Article

Guo-Jun Jiang¹  Guo-Zhen Shi¹  Yong-Fei Tan²  Zhi-Jun Ge³  Jian Zhou³  De-Jun Gong⁴  Jing Tao⁴  Yang Yuan⁴  Sheng-Dong Huang⁵ 

[1] Department of Cardiothoracic Surgery, Yixing People's Hospital, Jiangsu, P.R. China;Department of Cardiothoracic Surgery, Yixing People's Hospital, Jiangsu, P.R. China;Institute of Cardiothoracic Surgery, Yixing People's Hospital, 75 Tongzhenguan Rd, Wuxi, Jiangsu, P.R. China;Department of Pathology, Yixing People's Hospital, Jiangsu, P.R. China;Institute of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China;Institute of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China;Institute of Cardiothoracic Surgery, Changhai Hospital, 168, Changhai Rd, Shanghai, P.R. China;

关键词: Esophageal Squamous Cell Carcinoma;    Pathological Grade;    Esophageal Squamous Cell Carcinoma Cell;    Esophageal Squamous Cell Carcinoma Patient;    Arsenic Trioxide;   

DOI  :  10.1186/1471-2407-12-97

 received in 2011-11-29, accepted in 2012-03-21,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundPrenylated Rab acceptor 1 domain family member 3 (PRAF3) is involved in the regulation of many cellular processes including apoptosis, migration and invasion. This study was conducted to investigate the effect of PRAF3 on apoptosis, migration and invasion in human esophageal squamous cell carcinoma (ESCC).MethodsThe expression of PRAF3 mRNA and protein in primary ESCC and the matched normal tissues (57cases) was determined by quantitative RT-PCR and Western blot. Immunohistochemical analysis of PRAF3 expression was carried out in paraffin-embedded sections of ESCC and correlated with clinical features. The role of PRAF3 in apoptosis, migration and invasion was studied in ESCC cell lines of Eca109 and TE-1 through the adenovirus mediated PRAF3 gene transfer. The effect of PRAF3 on apoptosis was analyzed by annexin V-FITC assay. The regulation of PRAF3 on migration was determined by transwell and wounding healing assay, while the cellular invasion was analyzed by matrigel-coated transwell assay.ResultsWe found that the expression of PRAF3 was significantly down-regulated in ESCC tissue compared with the matched normal tissue and was correlated with the clinical features of pathological grade, tumor stage and lymph node metastasis. Moreover, overexpression of PRAF3 induced cell apoptosis through both caspase-8 and caspase-9 dependent pathways, and inhibited cell migration and invasion by suppressing the activity of both MMP-2 and MMP-9 in human ESCC cell lines.ConclusionsOur data suggest that PRAF3 plays an important role in the regulation of tumor progression and metastasis and serves as a tumor suppressor in human ESCC. We propose that PRAF3 might be used as a potential therapeutic agent for human ESCC.

【 授权许可】

Unknown   
© Shi et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.