Effects of polyunsaturated fatty acids on the growth of gastric cancer cells in vitro

Lipids in Health and Disease

Research

Shengrong Shen¹  Jinfeng Dai¹  Wensheng Pan²  Junhui Shen³  Undurti N Das⁴ 

[1] Department of Food Science and Nutrition, School of Biosystems Engineering & Food Science, Zhejiang University, 310058, Hangzhou, China;Department of Gastroenterology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, 310009, Hangzhou, China;School of Medicine of Tongji University, 200092, Shanghai, China;UND Life Sciences, 13800 Fairhill Road, #321, 44120, Shaker Heights, OH, USA;School of Biotechnology, Jawaharlal Nehru Technological University, 533 003, Kakinada, India;Bio-Science Research Centre, Gayatri Vidya Parishad College of Engineering, 530 048, Visakhapatnam, India;

关键词: Polyunsaturated fatty acids;    Gastric cancer cells;    Oxidative stress;    de novo;    Lipid metabolites;   

DOI  :  10.1186/1476-511X-12-71

 received in 2013-01-30, accepted in 2013-04-19,  发布年份 2013

来源: Springer

【 摘 要 】

Polyunsaturated fatty acids (PUFAs) have tumoricidal action, though the exact mechanism of their action is not clear. The results of the present study showed that of all the fatty acids tested, linoleic acid (LA) and α-linolenic acid (ALA) were the most effective in suppressing the growth of normal gastric cells (GES1) at 180 and 200 μM, while gastric carcinoma cells (MGC and SGC) were inhibited at 200 μM. Arachidonic acid (AA) suppressed the growth of GES1, MGC and SGC cells and lower concentrations (120 and 160 μM) of AA were more effective against gastric carcinoma (MGC and SGC) cells compared to normal gastric cells (GES1). Paradoxically, both eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids though are more unsaturated than AA, were less effective compared with LA, ALA and AA in suppressing the growth of both normal and cancer cells. At the concentration used, methotrexate showed much less growth suppressive action compared to all the fatty acids tested. PUFAs-treated cells showed accumulation of lipid droplets. A close association was noted between apoptosis and lipid peroxides formed compared to the ability of normal and tumor cells to generate ROS (reactive oxygen species) and induce SOD (superoxide dismutase activity) in response to fatty acids tested and methotrexate. Both normal and tumor cells generated lipoxin A4 (LXA4) in response to supplementation of fatty acids and methotrexate though no significant correlation was noted between their ability to induce apoptosis and LXA4 formed. These results suggest that PUFAs induced apoptosis of normal gastric and gastric carcinoma cells could, partly, be attributed to lipid peroxidation process.

【 授权许可】

Unknown   
© Dai et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.