Blood metabolomic fingerprint is distinct in healthy coronary and in stenosing or microvascular ischemic heart disease

Journal of Translational Medicine

Research

Damiana Congia¹  Giorgio Lai²  Gianfranco De Candia²  Mauro Cadeddu²  Raimondo Pirisi²  Cristina Piras³  Luigi Atzori³  Emanuela Locci⁴  Federica Ascedu⁴  Christian Cadeddu Dessalvi⁴  Giuseppe Mercuro⁴  Martino Deidda⁵ 

[1] Cardiology Complex Unit, Azienda Osperaliera Brotzu, Cagliari, Italy;Cath Lab, Azienda Ospedaliero-Universitaria di Cagliari, Cagliari, Italy;Department of Biomedical Sciences, University of Cagliari, Monserrato, Italy;Department of Medical Sciences and Public Health, University of Cagliari, Monserrato, Italy;Department of Medical Sciences and Public Health, University of Cagliari, Monserrato, Italy;Department of Medical Sciences and Public Health, University of Cagliari, Asse didattico Medicina, Cittadella Universitaria, SS Sestu KM 0.700, 09042, Monserrato, Italy;

关键词: Metabolomics;    Atherosclerosis;    Endothelial function;    Coronary artery;   

DOI  :  10.1186/s12967-017-1215-7

 received in 2017-02-10, accepted in 2017-05-17,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe endothelium is a key variable in the pathogenesis of atherosclerosis and its complications, particularly coronary artery disease (CAD). Current evidence suggests that the endothelial status can be regarded as an integrated index of individual atherogenic and anti-atherogenic properties, and that the interaction between circulating factors and the arterial wall might be critical for atherogenesis. In organism-level investigations, a functional view is provided by metabolomics, the study of the metabolic profile of small molecules. We sought to verify whether metabolomic analysis can reveal the presence of coronary microenvironment peculiarities associated with distinct manifestations of CAD.MethodsThirty-two coronary blood samples were analyzed using 1H-NMR-based metabolomics. Samples collected from patients with evidence of myocardial ischemia formed the case group, and were further divided into the stenotic-disease (SD) group (N = 13) and absence of stenosis (microvascular disease; “Micro”) group (N = 8); specimens of patients presenting no evidence of ischemic heart disease (dilated cardiomyopathy, valvular diseases) constituted the control group (N = 11).ResultsApplication of an orthogonal partial least squares discriminant analysis (OPLS-DA) model to the entire dataset clearly separated the samples into 3 groups, indicating 3 distinct metabolic fingerprints. Relative to control-group members, Micro patients showed a higher content of 2-hydroxybutirate, alanine, leucine, isoleucine, and N-acetyl groups and lower levels of creatine/phosphocreatine, creatinine, and glucose, whereas SD patients showed higher levels of 3-hydroxybutirate and acetate and a lower content of 2-hydroxybutirate. Moreover, relative to SD patients, Micro patients showed higher levels of 2-hydroxybutirate, alanine, leucine, and N-acetyl groups and lower levels of 3-hydroxybutirate and acetate.ConclusionsSpecific coronary microenvironments are likely associated with distinct development and pathological expression of CAD.

【 授权许可】

CC BY   
© The Author(s) 2017