p-Hydroxycinnamic acid production directly from cellulose using endoglucanase- and tyrosine ammonia lyase-expressing Streptomyces lividans

Microbial Cell Factories

Research

Tsutomu Tanaka¹  Yoshifumi Kawai¹  Chiaki Ogino¹  Shuhei Noda¹  Akihiko Kondo²  Naoko Okai³  Yasunobu Takeshima³ 

[1] Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, 657-8501, Nada, Kobe, Japan;Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, 657-8501, Nada, Kobe, Japan;Biomass Engineering Program, RIKEN, 1-7-22, 230-0045, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, Japan;Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, 657-8501, Nada, Kobe, Japan;

关键词: Streptomyces lividans;    P;    Endoglucanase;    Cellulose;   

DOI  :  10.1186/1475-2859-12-45

 received in 2013-02-26, accepted in 2013-04-14,  发布年份 2013

来源: Springer

【 摘 要 】

Backgroundp-Hydroxycinnamic acid (pHCA) is an aromatic compound that serves as a starting material for the production of many commercially valuable chemicals, such as fragrances and pharmaceuticals, and is also used in the synthesis of thermostable polymers. However, chemical synthesis of pHCA is both costly and harmful to the environment. Although pHCA production using microbes has been widely studied, there remains a need for more cost-effective methods, such as the use of biomass as a carbon source. In this study, we produced pHCA using tyrosine ammonia lyase-expressing Streptomyces lividans. In order to improve pHCA productivity from cellulose, we constructed a tyrosine ammonia lyase- and endoglucanase (EG)-expressing S. lividans transformant and used it to produce pHCA from cellulose.ResultsA Streptomyces lividans transformant was constructed to express tyrosine ammonia lyase derived from Rhodobacter sphaeroides (RsTAL). The transformant produced 786 or 736 mg/L of pHCA after 7 days of cultivation in medium containing 1% glucose or cellobiose as the carbon source, respectively. To enhance pHCA production from phosphoric acid swollen cellulose (PASC), we introduced the gene encoding EG into RsTAL-expressing S. lividans. After 7 days of cultivation, this transformant produced 753, 743, or 500 mg/L of pHCA from 1% glucose, cellobiose, or PASC, respectively.ConclusionsRsTAL-expressing S. lividans can produce pHCA from glucose and cellobiose. Similarly, RsTAL- and EG-expressing S. lividans can produce pHCA from glucose and cellobiose with excess EG activity remaining in the supernatant. This transformant demonstrated improved pHCA production from cellulose. Further enhancements in the cellulose degradation capability of the transformant will be necessary in order to achieve further improvements in pHCA production from cellulose.

【 授权许可】

Unknown   
© Kawai et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.