Integrated microRNA and whole-transcriptome sequencing reveals the involvement of small and long non-coding RNAs in the fiber growth of ramie plant

BMC Genomics

Research

Langbo Yi¹  Yanzhou Wang²  Siyuan Zhu²  Yafen Fu²  Chan Liu²  Jing Rao²  Xiai Yang²  Fu Li³  Touming Liu⁴ 

[1] College of Biology and Environmental Sciences, Jishou University, Jishou, China;Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China;Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China;College of Biology and Environmental Sciences, Jishou University, Jishou, China;Yangzhou University, Yangzhou, China;

关键词: Ramie;    Fiber growth;    Small RNA;    Long non-coding RNA;    MYB transcriptional factor;   

DOI  :  10.1186/s12864-023-09711-9

 received in 2023-02-07, accepted in 2023-10-03,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundMicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are the two main types of non-coding RNAs that play crucial roles in plant growth and development. However, their specific roles in the fiber growth of ramie plant (Boehmeria nivea L. Gaud) remain largely unknown.MethodsIn this study, we performed miRNA and whole-transcriptome sequencing of two stem bark sections exhibiting different fiber growth stages to determine the expression profiles of miRNAs, lncRNAs, and protein-encoding genes.ResultsAmong the identified 378 miRNAs and 6,839 lncRNAs, 88 miRNAs and 1,288 lncRNAs exhibited differential expression. Bioinformatics analysis revealed that 29 and 228 differentially expressed protein-encoding genes were targeted by differentially expressed miRNAs and lncRNAs, respectively, constituting eight putative competing endogenous RNA networks. lncR00022274 exhibited downregulated expression in barks with growing fibers. It also had an antisense overlap with the MYB gene, BntWG10016451, whose overexpression drastically increased the xylem fiber number and secondary wall thickness of fibers in the stems of transgenic Arabidopsis, suggesting the potential association of lncR00022274-BntWG10016451 expression with fiber growth.ConclusionsThese findings provide insights into the roles of ncRNAs in the regulation of fiber growth in ramie, which can be used for the biotechnological improvement of its fiber yield and quality in the future.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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