Endophytic microbiome of Boehmeria nivea and their antagonism against latent fungal pathogens in plants

BMC Microbiology

Research

Anne Nuraini¹  Mila Prasetyani²  Kartika Sari²  Erin Triani²  Asri Peni Wulandari³  Mohamad Nurzaman³  Rully Dyah Purwati⁴  Riksfardini A. Ermawar⁵ 

[1] Department of Agrotechnology, Faculty of Agriculture, Padjadjaran University, Sumedang, Indonesia;Department of Biology, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Sumedang, Indonesia;Department of Biology, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Sumedang, Indonesia;Center for Bioprospection of Natural Fibers and Biological Resources, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Sumedang, Indonesia;Research Center of Sweetener Plants and Fibers, Ministry of Agriculture, Jakarta, Indonesia;Research and Development Center of Biomaterials, National Research and Innovation Agency, Cibinong, Indonesia;

关键词: Antifungal;    Antagonistic interaction;    Endophyte;    Microbiome;    Ramie;   

DOI  :  10.1186/s12866-022-02737-1

 received in 2022-08-05, accepted in 2022-12-14,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundPathogenic microbes still become obstacles that can reduce the quality of plant growth, including ramie (Boehmeria nivea) plants. The study identified the microbiome and antagonistic interaction of the endophytic community from the B. nivea is necessary to improve the production of the ramie plant, especially ramie stem organs for fiber materials. ResultsTwenty isolates of endophytic microorganisms were obtained from the roots, stems, leaves, and flowers. They were identified using the Internal Transcribed Spacer (ITS) region of ribosomal (rDNA), and its morphotypes obtained 20 isolates, with a composition of 9 species of bacteria and 11 species of fungi. Besides that, the disease observations on ramie stems showed that four species of pathogenic fungi were identified as Fusarium solani isolate 3,248,941, Fusarium solani isolates colpat-359, Fusarium oxysporum isolate N-61–2, Clonostachys rosea strain B3042. The endophytic microorganism of ramie ability was tested to determine their potential to inhibit the growth of the pathogenic fungi based on the in-vivo antagonist test. The isolated bacteria were only able to inhibit the growth of F. solani, with the highest percentage of 54–55%. Three species of endophytic fungi, including Cladosporium tennissimum, Fusarium falciforme, and Penicillium citrinum, showed the best inhibition against the fungal pathogen Fusarium solani with the highest inhibitory presentation of 91–95%. Inhibitory interaction between the endophytic microbes and the ramie pathogens indicated the type of antibiosis, competition, and parasitism. ConclusionThe results of this study succeeded in showing the potential antifungal by endophytic fungi from ramie against the pathogens of the plant itself. P. citrinum isolate MEBP0017 showed the highest inhibition against all the pathogens of the ramie.

【 授权许可】

CC BY   
© The Author(s) 2022

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