Proteomic analysis of Salmonella enterica serovar Enteritidis following propionate adaptation

BMC Microbiology

Research Article

Leona N Calhoun¹  Young Min Kwon¹  Jackson O Lay²  Rohana Liyanage² 

[1] Cell and Molecular Biology Program, Department of Poultry Science, University of Arkansas, 1260 W. Maple Avenue, 72701, Fayetteville, AR, USA;State Wide Mass Spectrometry Facility, Chemistry and Biochemistry Department, University of Arkansas, 119 Chemistry Building, 72701, Fayetteville, AR, USA;

关键词: Acid Resistance;    Acid Stress;    Genetic Complementation;    Deletion Cassette;    Acid Shock;   

DOI  :  10.1186/1471-2180-10-249

 received in 2010-03-18, accepted in 2010-09-28,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundSalmonella Enteritidis is a highly prevalent and persistent foodborne pathogen and is therefore a leading cause of nontyphoidal gastrointestinal disease worldwide. A variety of stresses are endured throughout its infection cycle, including high concentrations of propionate (PA) within food processing systems and within the gut of infected hosts. Prolonged PA exposure experienced in such milieus may have a drastic effect on the proteome of Salmonella Enteritidis subjected to this stress.ResultsIn this study, we used 2 D gel electrophoresis to examine the proteomes of PA adapted and unadapted S. Enteritidis and have identified five proteins that are upregulated in PA adapted cultures using standard peptide mass fingerprinting by MALDI-TOF-MS and sequencing by MALDI LIFT-TOF/TOF tandem mass spectrometry. Of these five, two significant stress-related proteins (Dps and CpxR) were shown (via qRT-PCR analysis) to be upregulated at the transcriptional level as well. Unlike the wild type when adapted to PA (which demonstrates significant acid resistance), PA adapted S. Enteritidis ∆dps and S. Enteritidis ∆cpxR were at a clear disadvantage when challenged to a highly acidic environment. However, we found the acid resistance to be fully restorable after genetic complementation.ConclusionsThis work reveals a significant difference in the proteomes of PA adapted and unadapted S. Enteritidis and affirms the contribution of Dps and CpxR in PA induced acid resistance.

【 授权许可】

Unknown   
© Calhoun et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.