Decrypting the H-NS-dependent regulatory cascade of acid stress resistance in Escherichia coli

BMC Microbiology

Research Article

Antoine Danchin¹  Evelyne Krin²  Olga Soutourina³ 

[1] AMAbiotics 5, rue Henri Desbruères, 91030, Evry Cedex, France;Unité de Plasticité du Génome Bactérien, Institut Pasteur, France;Unité des Bactéries Anaérobies et Toxines, Institut Pasteur, France;Université Paris 7-Denis Diderot, 75205, Paris, France;

关键词: Agmatine;    Acid Resistance;    Acid Stress;    Glutamate Decarboxylase;    adiC Expression;   

DOI  :  10.1186/1471-2180-10-273

 received in 2010-06-02, accepted in 2010-10-29,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundH-NS regulates the acid stress resistance. The present study aimed to characterize the H-NS-dependent cascade governing the acid stress resistance pathways and to define the interplay between the different regulators.ResultsWe combined mutational, phenotypic and gene expression analyses, to unravel the regulatory hierarchy in acid resistance involving H-NS, RcsB-P/GadE complex, HdfR, CadC, AdiY regulators, and DNA-binding assays to separate direct effects from indirect ones. RcsB-P/GadE regulatory complex, the general direct regulator of glutamate-, arginine- and lysine-dependent acid resistance pathways plays a central role in the regulatory cascade. However, H-NS also directly controls specific regulators of these pathways (e.g. cadC) and genes involved in general stress resistance (hdeAB, hdeD, dps, adiY). Finally, we found that in addition to H-NS and RcsB, a third regulator, HdfR, inversely controls glutamate-dependent acid resistance pathway and motility.ConclusionsH-NS lies near the top of the hierarchy orchestrating acid response centred on RcsB-P/GadE regulatory complex, the general direct regulator of glutamate-, arginine- and lysine-dependent acid resistance pathways.

【 授权许可】

CC BY   
© Krin et al; licensee BioMed Central Ltd. 2010