Genomic insights into strategies used by Xanthomonas albilineans with its reduced artillery to spread within sugarcane xylem vessels

BMC Genomics

Research Article

Arnaud Couloux¹  Valérie Barbe¹  Béatrice Segurens¹  Sophie Mangenot¹  Monique Royer²  Isabelle Pieretti²  Philippe Rott²  Stéphane Cociancich³  Emmanuelle Lauber⁴  Armelle Darrasse⁵  Charles Manceau⁵  Marie-Agnès Jacques⁵  Sébastien Carrere⁶  Jérôme Gouzy⁶  Ralf Koebnik⁷  Boris Szurek⁷  Valérie Verdier⁷  Dean W Gabriel⁸  Matthieu Arlat⁹  Stéphane Poussier^1,10 

[1] CEA/DSV/IG/Génoscope, Centre National de Séquençage, F-91057, Evry, Cedex, France;CIRAD, UMR BGPI, F-34398, Montpellier, Cedex 5, France;CIRAD, UMR BGPI, F-34398, Montpellier, Cedex 5, France;UMR BGPI, Campus International de Baillarguet, TA A-54/K, F-34398, Montpellier, Cedex 5, France;CNRS, UMR LIPM, F-31326, Castanet-Tolosan, Cedex, France;INRA, UMR IRHS, F-49071, Beaucouzé, France;INRA, UMR LIPM, F-31326, Castanet-Tolosan, Cedex, France;IRD, UMR RPB, F-34394, Montpellier, Cedex 5, France;Plant Pathology Department, University of Florida, 32605, Gainesville, FL, USA;Université Paul Sabatier, UMR LIPM, F-31326, Castanet-Tolosan, Cedex, France;Université de la Réunion, UMR PVBMT, F-97715, Saint-Denis La Réunion, France;

关键词: Suppression Subtractive Hybridization;    Pathogenic Strain;    Cellulose Binding Domain;    NRPS Gene;    Xanthomonas Species;   

DOI  :  10.1186/1471-2164-13-658

 received in 2012-01-11, accepted in 2012-11-18,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundXanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. X. albilineans exhibits distinctive pathogenic mechanisms, ecology and taxonomy compared to other species of Xanthomonas. For example, this species produces a potent DNA gyrase inhibitor called albicidin that is largely responsible for inducing disease symptoms; its habitat is limited to xylem; and the species exhibits large variability. A first manuscript on the complete genome sequence of the highly pathogenic X. albilineans strain GPE PC73 focused exclusively on distinctive genomic features shared with Xylella fastidiosa—another xylem-limited Xanthomonadaceae. The present manuscript on the same genome sequence aims to describe all other pathogenicity-related genomic features of X. albilineans, and to compare, using suppression subtractive hybridization (SSH), genomic features of two strains differing in pathogenicity.ResultsComparative genomic analyses showed that most of the known pathogenicity factors from other Xanthomonas species are conserved in X. albilineans, with the notable absence of two major determinants of the “artillery” of other plant pathogenic species of Xanthomonas: the xanthan gum biosynthesis gene cluster, and the type III secretion system Hrp (hypersensitive response and pathogenicity). Genomic features specific to X. albilineans that may contribute to specific adaptation of this pathogen to sugarcane xylem vessels were also revealed. SSH experiments led to the identification of 20 genes common to three highly pathogenic strains but missing in a less pathogenic strain. These 20 genes, which include four ABC transporter genes, a methyl-accepting chemotaxis protein gene and an oxidoreductase gene, could play a key role in pathogenicity. With the exception of hypothetical proteins revealed by our comparative genomic analyses and SSH experiments, no genes potentially involved in any offensive or counter-defensive mechanism specific to X. albilineans were identified, supposing that X. albilineans has a reduced artillery compared to other pathogenic Xanthomonas species. Particular attention has therefore been given to genomic features specific to X. albilineans making it more capable of evading sugarcane surveillance systems or resisting sugarcane defense systems.ConclusionsThis study confirms that X. albilineans is a highly distinctive species within the genus Xanthomonas, and opens new perpectives towards a greater understanding of the pathogenicity of this destructive sugarcane pathogen.

【 授权许可】

Unknown   
© Pieretti et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.