Possible use of ail and foxA polymorphisms for detecting pathogenic Yersinia enterocolitica

BMC Microbiology

Research Article

Yuhuan Yang¹  Ying Huang²  Yuchun Xiao²  Jianguo Xu²  Huaiqi Jing²  Zhigang Cui²  Xin Wang²  Biao Kan²  Liuying Tang³ 

[1] Fujian Provincial Centre for Disease Control and Prevention, 350001, Fuzhou, China;National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, 102206, Beijing, China;National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100052, Beijing, China;

关键词: Reference Strain;    Sequence Pattern;    Pathogenic Strain;    Yersinia Enterocolitica;    Base Mutation;   

DOI  :  10.1186/1471-2180-10-211

 received in 2010-03-04, accepted in 2010-08-07,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundYersinia enterocolitica is an enteric pathogen that invades the intestinal mucosa and proliferates within the lymphoid follicles (Peyer's patches). The attachment invasion locus (ail) mediates invasion by Y. enterocolitica and confers an invasive phenotype upon non-invasive E. coli; ail is the primary virulence factor of Y. enterocolitica. The ferrioxamine receptor (foxA) located on the Y. enterocolitica chromosome, together with its transport protein, transports a siderophore specific for ferric ion. Currently, ail is the primary target gene for nucleic acid detection of pathogenic Y. enterocolitica.ResultsThe genes ail and foxA in 271 pathogenic and 27 non-pathogenic Y. enterocolitica strains isolated from China and 10 reference strains were sequenced, aligned, compared to the ail and foxA sequences of Yersinia enterocolitica subsp. enterocolitica 8081 (Genbank: NC_008800), and analyzed for sequence polymorphism. The ail from the 282 strains showed 3 sequence patterns: 277 strains of serotypes O:3, O:9 and O:5, 27 with identical nucleic acid sequences formed pattern A1; 4 strains of serotype 1B/O:8 with identical nucleic acid sequences formed pattern A2; and one Chinese isolate 2/O:9 formed pattern A3. In the primary coding region of the foxA ORF (Genebank: X60447 nt 433-1866; nt 28 to 1,461 in the ORF), the sequences formed 3 groups and were further divided into 8 sequence patterns.ConclusionThe ail and foxA loci of pathogenic Y. enterocolitica have been analyzed. The ail sequence was highly conserved among the same serotype strains from different sources; and foxA was highly conserved among the pathogenic strains, although there was some sequence diversity. Fewer strains were used from outside China, which is a limitation of the study.

【 授权许可】

Unknown   
© Huang et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.