High diversity of genetic lineages and virulence genes in nasal Staphylococcus aureusisolates from donkeys destined to food consumption in Tunisia with predominance of the ruminant associated CC133 lineage

BMC Veterinary Research

Research Article

Elena Gómez-Sanz¹  Carmen Lozano¹  Carmen Torres¹  Myriam Zarazaga¹  Ahlem Jouini²  Abdellatif Boudabous²  Haythem Gharsa²  Karim Ben Slama²  Rym Ben Sallem²  Naouel Klibi² 

[1] Departamento de Agricultura y Alimentación, Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Madre de Dios 51, 26006, Logroño, Spain;Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis-El Manar, 2092, Tunis, Tunisia;

关键词: Staphylococcus aureus;    Donkey;    spa;    CC133;    Virulence genes;    Tunisia;   

DOI  :  10.1186/1746-6148-8-203

 received in 2012-05-31, accepted in 2012-10-22,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe objective of this study was to determine the genetic lineages and the incidence of antibiotic resistance and virulence determinants of nasal Staphylococcus aureus isolates of healthy donkeys destined to food consumption in Tunisia.ResultsNasal swabs of 100 donkeys obtained in a large slaughterhouse in 2010 were inoculated in specific media for S. aureus and methicillin-resistant S. aureus (MRSA) recovery. S. aureus was obtained in 50% of the samples, being all of isolates methicillin-susceptible (MSSA). Genetic lineages, toxin gene profile, and antibiotic resistance mechanisms were determined in recovered isolates. Twenty-five different spa-types were detected among the 50 MSSA with 9 novel spa-types. S. aureus isolates were ascribed to agr type I (37 isolates), III (7), II (4), and IV (2). Sixteen different sequence-types (STs) were revealed by MLST, with seven new ones. STs belonging to clonal clomplex CC133 were majority. The gene tst was detected in 6 isolates and the gene etb in one isolate. Different combinations of enterotoxin, leukocidin and haemolysin genes were identified among S. aureus isolates. The egc-cluster-like and an incomplete egc-cluster-like were detected. Isolates resistant to penicillin, erythromycin, fusidic acid, streptomycin, ciprofloxacin, clindamycin, tetracycline, or chloramphenicol were found and the genes blaZ, erm(A), erm(C), tet(M), fusC were identified.ConclusionsThe nares of donkeys frequently harbor MSSA. They could be reservoirs of the ruminant-associated CC133 lineage and of toxin genes encoding TSST-1 and other virulence traits with potential implications in public health. CC133 seems to have a broader host distribution than expected.

【 授权许可】

Unknown   
© Gharsa et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.