Role of tissue transglutaminase 2 in the acquisition of a mesenchymal-like phenotype in highly invasive A431 tumor cells

Molecular Cancer

Research

Chithan C Kandaswami¹  Pei-Hsun Tsai²  Geen-Dong Chang²  Chang-Jen Huang³  Chun-Yu Lin³  Ming-Ting Lee³  Ping-Ping Lee⁴  Chia-Hsiung Cheng⁴  Jiuan-Jiuan Hwang⁵ 

[1] Castle Hills Health, 2267 Sir Amant Drive, 75056, Lewisville, TX, USA;Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan;Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan;Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan;Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan;Institute of Physiology, National Yang-Ming University, Taipei, Taiwan;

关键词: epithelial-mesenchymal transition;    tissue transglutaminase;    matrix metalloproteinase;    PI3K/Akt;    NF-κB;    Snail;    migration;   

DOI  :  10.1186/1476-4598-10-87

 received in 2011-03-09, accepted in 2011-07-21,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundCancer progression is closely linked to the epithelial-mesenchymal transition (EMT) process. Studies have shown that there is increased expression of tissue tranglutaminase (TG2) in advanced invasive cancer cells. TG2 catalyzes the covalent cross-linking of proteins, exhibits G protein activity, and has been implicated in the modulation of cell adhesion, migration, invasion and cancer metastasis. This study explores the molecular mechanisms associated with TG2's involvement in the acquisition of the mesenchymal phenotype using the highly invasive A431-III subline and its parental A431-P cells.ResultsThe A431-III tumor subline displays increased expression of TG2. This is accompanied by enhanced expression of the mesenchymal phenotype, and this expression is reversed by knockdown of endogenous TG2. Consistent with this, overexpression of TG2 in A431-P cells advanced the EMT process. Furthermore, TG2 induced the PI3K/Akt activation and GSK3β inactivation in A431 tumor cells and this increased Snail and MMP-9 expression resulting in higher cell motility. TG2 also upregulated NF-κB activity, which also enhanced Snail and MMP-9 expression resulting in greater cell motility; interestingly, this was associated with the formation of a TG2/NF-κB complex. TG2 facilitated acquisition of a mesenchymal phenotype, which was reversed by inhibitors of PI3K, GSK3 and NF-κB.ConclusionsThis study reveals that TG2 acts, at least in part, through activation of the PI3K/Akt and NF-κB signaling systems, which then induce the key mediators Snail and MMP-9 that facilitate the attainment of a mesenchymal phenotype. These findings support the possibility that TG2 is a promising target for cancer therapy.

【 授权许可】

Unknown   
© Lin et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.