Interaction of tissue transglutaminase with nuclear transport protein importin‐α3

FEBS Letters

Levitt, Mark L.²  Williams, James F.³  Lokshin, Anna⁴  Zhang, Haifan²  Graner, Scott¹  Peng, Xiaojun⁴  Zhang, Yonghui³ 

[1] Department of Pathology, Allegheny University of the Health Sciences, 320 E. North Ave, Pittsburgh, PA 15212, USA;Lung Cancer Program, Allegheny University of the Health Sciences, 320 E. North Ave, Pittsburgh, PA 15212, USA;Department of Biological Sciences, Carnegie Mellon University, Fifth Ave, Pittsburgh, PA 15213, USA;Magee-Womens Research Institute, Lab 320, 204 Craft Ave, Pittsburgh, PA 15213, USA

关键词: Tissue transglutaminase;    Importin;    Nuclear transport;    tTG;    tissue transglutaminase;    NSCLC;    non-small cell lung cancer;    RA;    all-trans retinoic acid;    EM;    electron microscopy;   

DOI  :  10.1016/S0014-5793(99)00018-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tissue transglutaminase is a multifunctional enzyme which has been involved in the regulation of cell growth, differentiation, and apoptosis. Recently, nuclear localization of tTG has been reported indicating the potential of active nuclear transport. In this study we use the yeast two-hybrid assay and co-immunoprecipitation to show that tTG interacts with the nuclear transport protein importin-α3. Using electron microscopy we demonstrate that nuclear expression of tTG in a non-small cell lung cancer cell line is induced by retinoic acid (RA). These data suggest that importin-α3 could mediate active nuclear transport of tTG which may be important for the regulation of critical cellular processes.

【 授权许可】

Unknown