Significantly reduced CCR5-tropic HIV-1 replication in vitro in cells from subjects previously immunized with Vaccinia Virus

BMC Immunology

Research Article

Kenneth Alibek¹  Raymond S Weinstein²  Michael M Weinstein³  Michael I Bukrinsky⁴  Beda Brichacek⁵ 

[1] AFG Biosolutions, 9119 Gaither Rd, 20877, Gaithersberg, MD, USA;Biodefense Program, Department of Public and International Affairs, George Mason University, 10900 University Blvd, MS 1H8, 20110, Manassas, VA, USA;Department of Human Genetics, University of California at Los Angeles, Gonda (Goldschmied) Neuroscience and Genetics Research Center, Box 708822, 695 Charles E. Young Drive South, 90095, Los Angeles, CA, USA;Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, Ross Hall, Suite 734, 2300 Eye St, 20037, N. W., Washington, DC, USA;Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, Ross Hall, Suite 734, 2300 Eye St, 20037, N. W., Washington, DC, USA;Current address: National Cancer Institute, National Institutes of Health, Bethesda, MD, USA;

关键词: Human Immunodeficiency Virus;    Vaccinia;    Smallpox;    Autologous Serum;    Human Immunodeficiency Virus Replication;   

DOI  :  10.1186/1471-2172-11-23

 received in 2009-10-23, accepted in 2010-05-18,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundAt present, the relatively sudden appearance and explosive spread of HIV throughout Africa and around the world beginning in the 1950s has never been adequately explained. Theorizing that this phenomenon may be somehow related to the eradication of smallpox followed by the cessation of vaccinia immunization, we undertook a comparison of HIV-1 susceptibility in the peripheral blood mononuclear cells from subjects immunized with the vaccinia virus to those from vaccinia naive donors.ResultsVaccinia immunization in the preceding 3-6 months resulted in an up to 5-fold reduction in CCR5-tropic but not in CXCR4-tropic HIV-1 replication in the cells from vaccinated subjects. The addition of autologous serum to the cell cultures resulted in enhanced R5 HIV-1 replication in the cells from unvaccinated, but not vaccinated subjects. There were no significant differences in the concentrations of MIP-1α, MIP-1β and RANTES between the cell cultures derived from vaccinated and unvaccinated subjects when measured in culture medium on days 2 and 5 following R5 HIV-1 challenge.DiscussionSince primary HIV-1 infections are caused almost exclusively by the CCR5-tropic HIV-1 strains, our results suggest that prior immunization with vaccinia virus might provide an individual with some degree of protection to subsequent HIV infection and/or progression. The duration of such protection remains to be determined. A differential elaboration of MIP-1α, MIP-1β and RANTES between vaccinated and unvaccinated subjects, following infection, does not appear to be a mechanism in the noted protection.

【 授权许可】

CC BY   
© Weinstein et al; licensee BioMed Central Ltd. 2010