Inflammation and adipose tissue: effects of progressive load training in rats

Lipids in Health and Disease

Research

Marília Seelaender¹  Ronaldo VT Santos²  Victor AF Tarini³  Flávio Faloppa³  Cláudia O do Nascimento⁴  Gustavo D Pimentel⁴  Eduardo S Alves⁴  Lila M Oyama⁴  Fábio S Lira⁴  José C Rosa⁵  Ricardo M Arida⁶  Marco T de Mello⁷ 

[1] Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil;Department of Bioscience, Universidade Federal de São Paulo, Baixada Santista Campus, São Paulo, Brazil;Department of Psychobiology, Universidade Federal de São Paulo - UNIFESP, São Paulo, Brazil;Department of Orthopaedics and Traumatology, Universidade Federal de São Paulo, São Paulo, Brazil;Department of Physiology of Nutrition, Universidade Federal de São Paulo, São Paulo, Brazil;Department of Physiology of Nutrition, Universidade Federal de São Paulo, São Paulo, Brazil;Department of Psychobiology, Universidade Federal de São Paulo - UNIFESP, São Paulo, Brazil;Department of Physiology, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil;Department of Psychobiology, Universidade Federal de São Paulo - UNIFESP, São Paulo, Brazil;

关键词: Adipose Tissue;    Corticosterone;    White Adipose Tissue;    Trained Group;    Hormone Sensitive Lipase;   

DOI  :  10.1186/1476-511X-9-109

 received in 2010-09-02, accepted in 2010-10-04,  发布年份 2010

来源: Springer

【 摘 要 】

IntroductionCytokines (IL-6, IL-10 and TNF-α) are increased after exhaustive exercise in the rat retroperitoneal (RPAT) and mesenteric adipose tissue (MEAT) pads. On the other hand, these cytokines show decreased expression in these depots in response to a chronic exercise protocol. However, the effect of exercise with overload combined with a short recovery period on pro- and anti-inflammatory cytokine expression is unknown. In the present study, we investigated the regulation of cytokine production in the adipose tissue of rats after an overtraining-inducing exercise protocol.MethodsMale Wistar rats were divided into four groups: Control (C), Trained (Tr), Overtrained (OT) and recovered overtrained (R). Cytokines (IL-6, TNF-α and IL-10) levels and Toll Like Receptor 4 (TLR4), Nuclear Factor kBp65 (NF-kBp65), Hormone Sensitive Lipase (HSL) and, Perilipin protein expression were assessed in the adipose tissue. Furthermore, we analysed plasma lipid profile, insulin, testosterone, corticosterone and endotoxin levels, and liver triacylglycerol, cytokine content, as well as apolipoprotein B (apoB) and TLR4 expression in the liver.ResultsOT and R groups exhibited reduced performance accompanied by lower testosterone and increased corticosterone and endotoxin levels when compared with the control and trained groups. IL-6 and IL-10 protein levels were increased in the adipose tissue of the group allowed to recover, in comparison with all the other studied groups. TLR-4 and NF-kBp65 were increased in this same group when compared with both control and trained groups. The protein expression of HSL was increased and that of Perilipin, decreased in the adipose in R in relation to the control. In addition, we found increased liver and serum TAG, along with reduced apoB protein expression and IL-6 and IL-10 levels in the of R in relation to the control and trained groups.ConclusionIn conclusion, we have shown that increases in pro-inflammatory cytokines in the adipose tissue after an overtraining protocol may be mediated via TLR-4 and NF-kBp65 signalling, leading to an inflammatory state in this tissue.

【 授权许可】

CC BY   
© Lira et al; licensee BioMed Central Ltd. 2010