Sleep deprivation affects inflammatory marker expression in adipose tissue

Lipids in Health and Disease

Research

Ronaldo VT Santos¹  Cláudia M Oller do Nascimento²  Cláudio A Cunha²  José C Rosa Neto²  Gustavo D Pimentel²  Fábio S Lira²  Lila M Oyama²  Daniel P Venancio³  Sérgio Tufik⁴  Marco T de Mello⁴ 

[1] Departmento de Biociências, Universidade Federal de São Paulo (UNIFESP), Baixada Santista Campus, São Paulo/SP, Brazil;Departmento de Fisiologia da Nutrição, Universidade Federal de São Paulo (UNIFESP), São Paulo/SP, Brazil;Departmento de Psicobiologia, Universidade Federal de São Paulo (UNIFESP), São Paulo/SP, Brazil;Departmento de Psicobiologia, Universidade Federal de São Paulo (UNIFESP), São Paulo/SP, Brazil;Associação de Suporte da Psicofarmacologia (AFIP), São Paulo/SP, Brazil;

关键词: Adipose Tissue;    Corticosterone;    Sleep Deprivation;    White Adipose Tissue;    Sleep Loss;   

DOI  :  10.1186/1476-511X-9-125

 received in 2010-06-24, accepted in 2010-10-30,  发布年份 2010

来源: Springer

【 摘 要 】

Sleep deprivation has been shown to increase inflammatory markers in rat sera and peripheral blood mononuclear cells. Inflammation is a condition associated with pathologies such as obesity, cancer, and cardiovascular diseases. We investigated changes in the pro and anti-inflammatory cytokines and adipokines in different depots of white adipose tissue in rats. We also assessed lipid profiles and serum levels of corticosterone, leptin, and adiponectin after 96 hours of sleep deprivation.MethodsThe study consisted of two groups: a control (C) group and a paradoxical sleep deprivation by 96 h (PSD) group. Ten rats were randomly assigned to either the control group (C) or the PSD. Mesenteric (MEAT) and retroperitoneal (RPAT) adipose tissue, liver and serum were collected following completion of the PSD protocol. Levels of interleukin (IL)-6, interleukin (IL)-10 and tumour necrosis factor (TNF)-α were analysed in MEAT and RPAT, and leptin, adiponectin, glucose, corticosterone and lipid profile levels were analysed in serum.ResultsIL-6 levels were elevated in RPAT but remained unchanged in MEAT after PSD. IL-10 protein concentration was not altered in either depot, and TNF-α levels decreased in MEAT. Glucose, triglycerides (TG), VLDL and leptin decreased in serum after 96 hours of PSD; adiponectin was not altered and corticosterone was increased.ConclusionPSD decreased fat mass and may modulate the cytokine content in different depots of adipose tissue. The inflammatory response was diminished in both depots of adipose tissue, with increased IL-6 levels in RPAT and decreased TNF-α protein concentrations in MEAT and increased levels of corticosterone in serum.

【 授权许可】

CC BY   
© Rosa Neto et al; licensee BioMed Central Ltd. 2010