Overexpression of the grapevine PGIP1 in tobacco results in compositional changes in the leaf arabinoxyloglucan network in the absence of fungal infection

BMC Plant Biology

Research Article

Annatjie Hugo¹  William GT Willats²  Jonatan U Fangel²  Alexandra D Fagerström³  Melané A Vivier⁴  John P Moore⁴  Eric Nguema-Ona⁵ 

[1] Department of Microbiology, Stellenbosch University, 7602, Matieland, South Africa;Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, 1001, DK-, Denmark;Energy Biosciences Institute, University of California, 2151 Berkeley Way, 94720-5230, Berkeley, CA, USA;Institute for Wine Biotechnology, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, 7602, Matieland, South Africa;Institute for Wine Biotechnology, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, 7602, Matieland, South Africa;Laboratoire Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV). Grand Réseau de Recherche VASI de Haute Normandie, PRES Normandie Université. Université de Rouen, 76821, Mont Saint Aignan, Cedex, France;

关键词: Tobacco;    Grapevine;    Cell wall;    Profiling;    VvPGIP1;    Polygalacturonase-inhibiting protein (PGIP);    Endopolygalacturonase (ePG);    Arabinoxyloglucan (AXyG);    Xyloglucan-specific endoglucanase (XEG);    Arabinogalactan proteins (AGPs);   

DOI  :  10.1186/1471-2229-13-46

 received in 2012-09-05, accepted in 2013-02-07,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundConstitutive expression of Vitis vinifera polygalacturonase-inhibiting protein 1 (Vvpgip1) has been shown to protect tobacco plants against Botrytis cinerea. Evidence points to additional roles for VvPGIP1, beyond the classical endopolygalacturonase (ePG) inhibition mechanism, in providing protection against fungal infection. Gene expression and biochemical datasets previously obtained, in the absence of infection, point to the cell wall, and particularly the xyloglucan component of transgenic VvPGIP1 lines as playing a role in fungal resistance.ResultsTo elucidate the role of wall-associated processes in PGIP-derived resistance pre-infection, a wall profiling analysis, using high-throughput and fractionation techniques, was performed on healthy leaves from wild-type and previously characterized transgenic lines. The cell wall structure profile during development was found to be altered in the transgenic lines assessed versus the wild-type plants. Immunoprofiling revealed subtle changes in pectin and cellulose components and marked changes in the hemicellulose matrix, which showed reduced binding in transgenic leaves of VvPGIP1 expressing plants. Using an enzymatic xyloglucan oligosaccharide fingerprinting technique optimized for tobacco arabinoxyloglucans, we showed that polysaccharides of the XEG-soluble domain were modified in relative abundance for certain oligosaccharide components, although no differences in ion profiles were evident between wild-type and transgenic plants. These changes did not significantly influence plant morphology or normal growth processes compared to wild-type lines.ConclusionsVvPGIP1 overexpression therefore results in cell wall remodeling and reorganization of the cellulose-xyloglucan network in tobacco in advance of potential infection.

【 授权许可】

Unknown   
© Nguema-Ona et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.