Gum arabic modified Fe3O4 nanoparticles cross linked with collagen for isolation of bacteria

Journal of Nanobiotechnology

Research

Ashwin Murugappan Chockalingam¹  Heman Kumar Ramiya Ramesh Babu²  Raghuraman Chittor²  Jai Prakash Tiwari³ 

[1] Centre for Education, Central Electrochemical Research Institute, 630006, Karaikudi, Tamilnadu, India;Department of Biotechnology, Kamaraj College of Engineering and Technology, 626001, Virudhunagar, Tamilnadu, India;Functional Materials Division, Central Electrochemical Research Institute, 630006, Karaikudi, Tamilnadu, India;

关键词: Fe3O4;    Magnetic Nanoparticles;    Fe3O4 Nanoparticles;    Iron Oxide Nanoparticles;    FePt;   

DOI  :  10.1186/1477-3155-8-30

 received in 2010-09-02, accepted in 2010-12-15,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundMultifunctional magnetic nanoparticles are important class of materials in the field of nanobiotechnology, as it is an emerging area of research for material science and molecular biology researchers. One of the various methods to obtain multifunctional nanomaterials, molecular functionalization by attaching organic functional groups to nanomagnetic materials is an important technique. Recently, functionalized magnetic nanoparticles have been demonstrated to be useful in isolation/detection of dangerous pathogens (bacteria/viruses) for human life. Iron (Fe) based material especially FePt is used in the isolation of ultralow concentrations (< 102 cfu/ml) of bacteria in less time and it has been demonstrated that van-FePt may be used as an alternative fast detection technique with respect to conventional polymerase chain reaction (PCR) method. However, still further improved demonstrations are necessary with interest to biocompatibility and green chemistry. Herein, we report the synthesis of Fe3O4 nanoparticles by template medication and its application for the detection/isolation of S. aureus bacteria.ResultsThe reduction of anhydrous Iron chloride (FeCl3) in presence of sodium borohydride and water soluble polyelectrolyte (polydiallyldimethyl ammonium chloride, PDADMAC) produces black precipitates. The X-ray diffraction (XRD), XPS and TEM analysis of the precipitates dried at 373 K demonstrated the formation of nanocrystalline Fe3O4. Moreover, scanning electron microscopy (SEM) showed isolated staphylococcous aureus (S. aureus) bacteria at ultralow concentrations using collagen coated gum arabic modified iron oxide nanoparticles (CCGAMION).ConclusionWe are able to synthesize nanocrystalline Fe3O4 and CCGAMION was able to isolate S. aureus bacteria at 8-10 cfu (colony forming units)/ml within ~3 minutes.

【 授权许可】

Unknown   
© Chockalingam et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.