In situ detection of GM1 and GM2 gangliosides using immunohistochemical and immunofluorescent techniques for auxiliary diagnosis of canine and feline gangliosidoses

BMC Veterinary Research

Methodology Article

Kimimasa Takahashi¹  Daisuke Hasegawa²  Yuya Nakamoto³  Osamu Yamato⁴  Moeko Kohyama⁴  Akira Yabuki⁴  Hiroaki Kawaguchi⁵  Kenji Ochiai⁶  Masaya Tsuboi⁷  Kazuyuki Uchida⁷ 

[1] Department of Veterinary Pathology, Nippon Veterinary and Life Science University, 1-7-1 Kyouman-chou, Musashino-shi, Tokyo, 180-8602, Japan;Department of Veterinary Radiology, Nippon Veterinary and Life Science University, 1-7-1 Kyouman-chou, Musashino-shi, 180-8602, Tokyo, Japan;Kyoto Animal Referral Medical Center, 208-4 Shin-arami, Tai, Kumiyama-cho, Kuse-gun, 613-0036, Kyoto, Japan;Laboratory of Clinical Pathology, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima-shi, 890-0065, Kagoshima, Japan;Laboratory of Veterinary Histopathology, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima-shi, 890-0065, Kagoshima, Japan;Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka-shi, 020-8550, Iwate, Japan;Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyou-ku, 113-8657, Tokyo, Japan;

关键词: Gangliosidosis;    Dog;    Cat;    Lysosomal Storage Disease;    Immunohistochemistry;    Immunofluorescence;   

DOI  :  10.1186/s12917-016-0691-y

 received in 2015-12-31, accepted in 2016-03-22,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundGM1 and GM2 gangliosidoses are progressive neurodegenerative lysosomal storage diseases resulting from the excessive accumulation of GM1 and GM2 gangliosides in the lysosomes, respectively. The diagnosis of gangliosidosis is carried out based on comprehensive findings using various types of specimens for histological, ultrastructural, biochemical and genetic analyses. Therefore, the partial absence or lack of specimens might have resulted in many undiagnosed cases. The aim of the present study was to establish immunohistochemical and immunofluorescent techniques for the auxiliary diagnosis of canine and feline gangliosidoses, using paraffin-embedded brain specimens stored for a long period.ResultsUsing hematoxylin and eosin staining, cytoplasmic accumulation of pale to eosinophilic granular materials in swollen neurons was observed in animals previously diagnosed with GM1 or GM2 gangliosidosis. The immunohistochemical and immunofluorescent techniques developed in this study clearly demonstrated the accumulated material to be either GM1 or GM2 ganglioside.ConclusionsImmunohistochemical and immunofluorescent techniques using stored paraffin-embedded brain specimens are useful for the retrospective diagnosis of GM1 and GM2 gangliosidoses in dogs and cats.

【 授权许可】

CC BY   
© Kohyama et al. 2016