Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reaction

Journal of venomous animals and toxins

lio Langoni¹  Simone Baldini Lucheis²  Campos Braga³  Audrey Rennó⁴  Hé⁷ 

[1] Univ Estadual Paulista), Botucatu, Brazil;AgêDepartment of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Husbandry, SãMineiro Institute of Agriculture (IMA), Uberaba, Brazil;cios (APTA/SAA), Polo Regional Centro-Oeste, Bauru, Brazil;ncia Paulista de Tecnologia dos Agronegóo Paulo State University (UNESP –

关键词: Dog;    Cat;    Diagnosis;    Leishmaniasis;    Zoonosis;   

DOI  :  10.1186/1678-9199-20-5

学科分类：药理学

来源: BioMed Central

【 摘 要 】

This study aimed to evaluate the occurrence of Leishmania spp. in dogs and cats from Botucatu, São Paulo state, and Campo Grande, Mato Grosso do Sul state, Brazil, by the association of three diagnostic tests: blood culture in liver infusion tryptose medium, immunofluorescent antibody test and polymerase chain reaction. Fifty blood samples of dogs and cats from the Center for Zoonosis Control in Campo Grande, an area endemic for canine visceral leishmaniasis, were collected randomly, as well as canine and feline blood samples from the Municipal Kennel and Animal Protection Association in Botucatu, currently considered a transmission-free, non-endemic area. Of the 50 dog blood cultures from Botucatu, three (6%) were positive and of the 50 cats, two (4%) were positive. In Campo Grande, 29 dog blood cultures (58%) were positive and all (100%) cats negative by this test. Polymerase chain reaction detected Leishmania spp. in 100% of dog and cat samples from Botucatu but found all the cats from Campo Grande to be negative. On the other hand, 36 dogs from Campo Grande were positive (72%) by the same technique. Immunofluorescent antibody test in Botucatu found 100% of dogs and cats non-reactive, while in Campo Grande, it detected positivity in 32 dogs (64%) and 15 cats (30%). The results show the importance of not only continuous epidemiological surveillance in areas not endemic for leishmaniasis, but also research for accurate diagnosis of this zoonosis.

【 授权许可】

CC BY