Haemophilus influenzae porine omp P2 gene transfer mediated by graphene oxide nanoparticles with effects on transformation process and virulence bacterial capacity

Journal of Nanobiotechnology

Research

Rafaella Fabiana Carneiro Pereira¹  Marcelo Lancellotti¹  Cecília Cecília Krähenbühl Amstalden¹  Luciana Maria de Hollanda¹  Julia Nogueira Varela¹  Vitor Baranauskas²  Helder José Ceragioli² 

[1] LABIOTEC - Biotechnology Laboratory, Department of Biochemistry, Institute of Biology CP6109, University of Campinas - UNICAMP 13083-970, Campinas, São Paulo, Brazil;NanoEng - NanoEngineering and Diamond Laboratory, Department of Semiconductors, Instruments and Photonics, School of Electrical and Computer Engineering, University of Campinas, UNICAMP, Av. Albert Einstein N.400, CEP 13 083-852 Campinas, São Paulo, Brazil;

关键词: Haemophilus influenzae;    Graphene oxide;    Bacterial transformation;    Outer membrane protein;    Virulence;    Porin;   

DOI  :  10.1186/1477-3155-12-14

 received in 2014-01-15, accepted in 2014-04-04,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundH. influenzae is a natural competent bacterium that can uptake DNA from the environment and recombine into bacterial genome. The outbreaks of Brazilian purpuric fever, heavily polluted areas of a different H. influenzae biogroup - aegyptius - as well as gene transference between Neisseria meningitis make the transformation process an important evolutionary factor. This work studied the horizontal transference of the omp P2 gene from a multiresistant strain of H. influenzae 07 (NTHi), under the influence of graphene oxide nanoparticles in order to mimic an atmosphere rich in suspended particles and this way verify if the CFU transformants number was increased.Material and methodsIn this article the gene ompP2 was transformed into different strains of H. influenzae mediated or not by graphene oxide nanoparticles in suspension, followed by the adhesion tests in Hec-1B (human endometrium adenocarcinoma) and A549 (pulmonary epithelial carcinoma) cells lines. The transformation frequency and the adhesion capacity were determined in all the mutants to which the ompP2 gene was transferred and compared to their wild type strains.ResultsThe nanoparticles increased the transformation ratio of one particular strain isolated from a pneumonia case. The adhesion patterns to A549 and Hec1b cell lines of these mutated bacteria has their capacity increased when compared to the wild type.ConclusionsGraphene oxide nanoparticles aid the transformation process, helping to increase the number of CFUs, and the mutants generated with the omp P2 gene from a H. influenzae resistant strain not only present a chloramphenicol resistance but also have an increased adherence patterns in A549 and Hec1B cell lines.

【 授权许可】

Unknown   
© Varela et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.