Development of rapid microwave-mediated and low-temperature bacterial transformations

Journal of Chemical Biology

Valerie T. Tripp¹  Douglas D. Young¹  Johnathan C. Maza¹ 

[1] Department of Chemistry, The College of William & Mary, Williamsburg, VA 23187 USA

关键词: Microwave irradiation;    Bacterial transformation;    Molecular biology;   

DOI  :  10.1007/s12154-013-0095-4

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

The introduction of exogenous DNA into Escherichia coli is a cornerstone of molecular biology. Herein, we investigate two new mechanisms for bacterial transformation involving either the use of microwave irradiation or a freeze–thaw protocol in liquid nitrogen. Ultimately, both methods afforded successful transfer of plasmid DNA into bacterial cells, with the freeze–thaw technique yielding efficiencies of ~105. More importantly, both techniques effectively eliminated the need for the preparation of competent cells.

【 授权许可】

Unknown