Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.)

BMC Plant Biology

Research Article

Nicola Zambrano¹  Andrea Scaloni²  Simona Arena²  Mauro Sassi²  Monica Vitale³  Nunzia Scotti⁴  Lorenza Sannino⁴  Alessandra Ruggiero⁴  Stefania Grillo⁴  Antonello Costa⁴  Rachele Tamburino⁴  Giorgia Batelli⁴ 

[1] Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, via Pansini, 80100, Napoli, Italy;Center of Genetics Engineering (CEINGE) Biotecnologie Avanzate S.c. a R.l, via Pansini, 80100, Napoli, Italy;Institute for the Animal Production System in the Mediterranean Environment, National Research Council of Italy (CNR-ISPAAM), via Argine 1085, 80147, Napoli, Italy;Institute for the Animal Production System in the Mediterranean Environment, National Research Council of Italy (CNR-ISPAAM), via Argine 1085, 80147, Napoli, Italy;Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, via Pansini, 80100, Napoli, Italy;Institute of Biosciences and BioResources, National Research Council of Italy (CNR-IBBR), via Università 133, 80055, Portici, NA, Italy;

关键词: Water deficit;    Proteomic analysis;    Abscisic acid;    Proline;    Environmental sensor;    Retrograde signaling;   

DOI  :  10.1186/s12870-017-0971-0

 received in 2016-05-21, accepted in 2017-01-04,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundDrought is a major constraint for plant growth and crop productivity that is receiving an increased attention due to global climate changes. Chloroplasts act as environmental sensors, however, only partial information is available on stress-induced mechanisms within plastids. Here, we investigated the chloroplast response to a severe drought treatment and a subsequent recovery cycle in tomato through physiological, metabolite and proteomic analyses.ResultsUnder stress conditions, tomato plants showed stunted growth, and elevated levels of proline, abscisic acid (ABA) and late embryogenesis abundant gene transcript. Proteomics revealed that water deficit deeply affects chloroplast protein repertoire (31 differentially represented components), mainly involving energy-related functional species. Following the rewatering cycle, physiological parameters and metabolite levels indicated a recovery of tomato plant functions, while proteomics revealed a still ongoing adjustment of the chloroplast protein repertoire, which was even wider than during the drought phase (54 components differentially represented). Changes in gene expression of candidate genes and accumulation of ABA suggested the activation under stress of a specific chloroplast-to-nucleus (retrograde) signaling pathway and interconnection with the ABA-dependent network.ConclusionsOur results give an original overview on the role of chloroplast as enviromental sensor by both coordinating the expression of nuclear-encoded plastid-localised proteins and mediating plant stress response. Although our data suggest the activation of a specific retrograde signaling pathway and interconnection with ABA signaling network in tomato, the involvement and fine regulation of such pathway need to be further investigated through the development and characterization of ad hoc designed plant mutants.

【 授权许可】

CC BY   
© The Author(s). 2017