Proteomic analysis and interactions network in leaves of mycorrhizal and nonmycorrhizal sorghum plants under water deficit

PeerJ

article

Víctor Olalde-Portugal¹  José Luis Cabrera-Ponce²  Argel Gastelum-Arellanez³  Armando Guerrero-Rangel¹  Robert Winkler¹  Silvia Valdés-Rodríguez¹ 

[1] Departamento de Biotecnología y Bioquímica;Departamento de Ingeniería Genética;Área de Medio Ambiente y Biotecnología

关键词: Water deficit;    Arbuscular mycorrhizal fungi;    Protein–protein interaction;    Gene network;    Ribosomal proteins;    ATP synthase;    Signaling;    Photosynthesis;    Proteomic analysis;    Sorghum;   

DOI  :  10.7717/peerj.8991

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

For understanding the water deficit stress mechanism in sorghum, we conducted a physiological and proteomic analysis in the leaves of Sorghum bicolor L. Moench (a drought tolerant crop model) of non-colonized and colonized plants with a consortium of arbuscular mycorrhizal fungi. Physiological results indicate that mycorrhizal fungi association enhances growth and photosynthesis in plants, under normal and water deficit conditions. 2D-electrophoresis profiles revealed 51 differentially accumulated proteins in response to water deficit, of which HPLC/MS successfully identified 49. Bioinformatics analysis of protein–protein interactions revealed the participation of different metabolic pathways in nonmycorrhizal compared to mycorrhizal sorghum plants under water deficit. In noninoculated plants, the altered proteins are related to protein synthesis and folding (50S ribosomal protein L1, 30S ribosomal protein S10, Nascent polypeptide-associated complex subunit alpha), coupled with multiple signal transduction pathways, guanine nucleotide-binding beta subunit (Rack1) and peptidyl-prolyl-cis-trans isomerase (ROC4). In contrast, in mycorrhizal plants, proteins related to energy metabolism (ATP synthase-24kDa, ATP synthase β), carbon metabolism (malate dehydrogenase, triosephosphate isomerase, sucrose-phosphatase), oxidative phosphorylation (mitochondrial-processing peptidase) and sulfur metabolism (thiosulfate/3-mercaptopyruvate sulfurtransferase) were found. Our results provide a set of proteins of different metabolic pathways involved in water deficit produced by sorghum plants alone or associated with a consortium of arbuscular mycorrhizal fungi isolated from the tropical rain forest Los Tuxtlas Veracruz, México.

【 授权许可】

CC BY