Effect of ischemic preconditioning in skeletal muscle measured by functional magnetic resonance imaging and spectroscopy: a randomized crossover trial

Journal of Cardiovascular Magnetic Resonance

Research

Ewald Moser¹  Martin Andreas²  Johann Bartko²  Michael Wolzt²  Daniel Doberer²  Albrecht I Schmid³  Mohammad Keilani⁴  Richard Crevenna⁴ 

[1] Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Waehringer Guertel 18-20, A-1090, Vienna, Austria;MR Center of Excellence, Medical University of Vienna, Waehringer Guertel 18-20, A-1090, Vienna, Austria;Department of Clinical Pharmacology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090, Vienna, Austria;Department of Clinical Pharmacology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090, Vienna, Austria;Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Waehringer Guertel 18-20, A-1090, Vienna, Austria;MR Center of Excellence, Medical University of Vienna, Waehringer Guertel 18-20, A-1090, Vienna, Austria;Department of Physical Medicine and Rehabilitation, Medical University of Vienna, Waehringer Guertel 18-20, A-1090, Vienna, Austria;

关键词: Nuclear Magnetic Resonance;    Ischemic Precondition;    Muscular Strength;    Plantar Flexion;    Bold Signal;   

DOI  :  10.1186/1532-429X-13-32

 received in 2011-02-15, accepted in 2011-06-30,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundNuclear magnetic resonance (NMR) imaging and spectroscopy have been applied to assess skeletal muscle oxidative metabolism. Therefore, in-vivo NMR may enable the characterization of ischemia-reperfusion injury. The goal of this study was to evaluate whether NMR could detect the effects of ischemic preconditioning (IPC) in healthy subjects.MethodsTwenty-three participants were included in two randomized crossover protocols in which the effects of IPC were measured by NMR and muscle force assessments. Leg ischemia was administered for 20 minutes with or without a subsequent impaired reperfusion for 5 minutes (stenosis model). IPC was administered 4 or 48 hours prior to ischemia. Changes in 31phosphate NMR spectroscopy and blood oxygen level-dependent (BOLD) signals were recorded. 3-Tesla NMR data were compared to those obtained for isometric muscular strength.ResultsThe phosphocreatine (PCr) signal decreased robustly during ischemia and recovered rapidly during reperfusion. In contrast to PCr, the recovery of muscular strength was slow. During post-ischemic stenosis, PCr increased only slightly. The BOLD signal intensity decreased during ischemia, ischemic exercise and post-ischemic stenosis but increased during hyperemic reperfusion. IPC 4 hours prior to ischemia significantly increased the maximal PCr reperfusion signal and mitigated the peak BOLD signal during reperfusion.ConclusionsIschemic preconditioning positively influenced muscle metabolism during reperfusion; this resulted in an increase in PCr production and higher oxygen consumption, thereby mitigating the peak BOLD signal. In addition, an impairment of energy replenishment during the low-flow reperfusion was detected in this model. Thus, functional NMR is capable of characterizing changes in reperfusion and in therapeutic interventions in vivo.Trial RegistrationClinicalTrials.gov: NCT00883467

【 授权许可】

Unknown   
© Andreas et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.