Emergence and clonal transmission of multi-drug-resistant tuberculosis among patients in Chad

BMC Infectious Diseases

Research Article

Colette Diguimbaye Djaibé¹  Seynabou Lo²  Gedeon W. Ossoga³  Barou Djouater³  Richard Ngandolo³  Souleymane Mboup⁴  Aissatou G. Diallo⁵  Awa Ba Diallo⁵  Bouke C. de Jong⁶  Geraldine Daneau⁶  Florian Gehre⁷ 

[1] Clinique Médico-Chirurgicale PROVIDENCE, N’Djamena, Chad;Faculty of Health Sciences, Gaston Berger University, Saint Louis, Senegal;Institut de Recherche en Elevage pour le Developpement, N’Djamena, Chad;Institut de Recherche en Santé, de Surveillance Epidemiologique et de Formation, Diamniadio, Senegal;Mycobacteria Unit, Bacteriology- Virology Laboratory, CHU Aristide Le Dantec, 30 Avenue Pasteur, BP 7325, Dakar, Senegal;Mycobacteriology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium;Mycobacteriology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium;Medical Reserach Council (MRC) Unit, Fajara, Gambia;

关键词: Mycobacterium tuberculosis;    MDR;    Molecular characterization;    Chad;   

DOI  :  10.1186/s12879-017-2671-7

 received in 2016-10-28, accepted in 2017-08-07,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundEmergence of Multidrug-resistant (MDR) strains constitutes a significant public health problem worldwide. Prevalence of MDR tuberculosis from Chad is unavailable to date.MethodsWe collected samples from consecutive TB patients nationwide in the seven major cities of Chad between 2007 and 2012 to characterize drug resistance and the population structure of circulating Mycobacterium tuberculosis complex (MTBC) strains. We tested drug sensitivity using Line Probe Assays and phenotypic drug susceptibility testing (DST) were used for second line drugs. We genotyped the isolates using spoligotype analysis and MIRU-VNTR.ResultsA total of 311 cultures were isolated from 593 patients. The MDR prevalence was 0.9% among new patients and 3.5% among retreatment patients, and no second line drug resistance was identified. The distribution of genotypes suggests a dissemination of MDR strains in the Southern city of Moundou, bordering Cameroon and Central African Republic.ConclusionEmerging MDR isolates pose a public health threat to Southern Chad, with risk to neighboring countries. This study informs public health practitioners, justifying the implementation of continuous surveillance with DST for all retreatment cases as well as contacts of MDR patients, in parallel with provision of adequate 2nd line regimens in the region.

【 授权许可】

CC BY   
© The Author(s). 2017